Universität zu Köln

Bakonyi, Daniel, Toelzer, Christine ORCID: 0000-0001-9984-5940, Stricker, Michael, Hummel, Werner, Niefind, Karsten ORCID: 0000-0002-0183-6315 and Groeger, Harald (2020). Expanding the Application Range of Microbial Oxidoreductases by an Alcohol Dehydrogenase from Comamonas testosteroni with a Broad Substrate Spectrum and pH Profile. Catalysts, 10 (11). BASEL: MDPI. ISSN 2073-4344

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Abstract

Alcohol dehydrogenases catalyse the conversion of a large variety of ketone substrates to the corresponding chiral products. Due to their high regio- and stereospecificity, they are key components in a wide range of industrial applications. A novel alcohol dehydrogenase from Comamonas testosteroni (CtADH) was identified in silico, recombinantly expressed and purified, enzymatically and biochemically investigated as well as structurally characterized. These studies revealed a broad pH profile and an extended substrate spectrum with the highest activity for compounds containing halogens as substituents and a moderate activity for bulky-bulky ketones. Biotransformations with selected ketones-performed with a coupled regeneration system for the co-substrate NADPH-resulted in conversions of more than 99% with all tested substrates and with excellent enantioselectivity for the corresponding S-alcohol products. CtADH/NADPH/substrate complexes modelled on the basis of crystal structures of CtADH and its closest homologue suggested preliminary hints to rationalize the enzyme's substrate preferences

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bakonyi, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Toelzer, Christine UNSPECIFIED orcid.org/0000-0001-9984-5940 UNSPECIFIED Stricker, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Hummel, Werner UNSPECIFIED UNSPECIFIED UNSPECIFIED Niefind, Karsten UNSPECIFIED orcid.org/0000-0002-0183-6315 UNSPECIFIED Groeger, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-313093
DOI:	10.3390/catal10111281
Journal or Publication Title:	Catalysts
Volume:	10
Number:	11
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4344
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BULKY-BULKY KETONES; BIOCHEMICAL-CHARACTERIZATION; GLUCOSE-DEHYDROGENASE; REDUCTION; RALSTONIA; INTERMEDIATE; BIOCATALYSIS; REGENERATION; RESOLUTION; FEATURES Multiple languages Chemistry, Physical Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/31309