Universität zu Köln

Kraemer, Bjoern F., Geimer, Marc, Franz-Wachtel, Mirita, Lamkemeyer, Tobias, Mannell, Hanna and Lindemann, Stephan (2020). Extracellular Matrix-Specific Platelet Activation Leads to a Differential Translational Response and Protein De Novo Synthesis in Human Platelets. Int. J. Mol. Sci., 21 (21). BASEL: MDPI. ISSN 1422-0067

Full text not available from this repository.

Abstract

Platelets are exposed to extracellular matrix (ECM) proteins like collagen and laminin and to fibrinogen during acute vascular events. However, beyond hemostasis, platelets have the important capacity to migrate on ECM surfaces, but the translational response of platelets to different extracellular matrix stimuli is still not fully characterized. Using 2D-gel electrophoresis, confocal microscopy, polysome analysis and protein sequencing by mass spectrometry, we demonstrate that platelets show a differential expression profile of newly synthesized proteins on laminin, collagen or fibrinogen. In this context, we observed a characteristic, ECM-dependent translocation phenotype of translation initiation factor eIF4E to the ribosomal site. eIF4E accumulated in polysomes with increased binding of mRNA and co-localization with vinculin, leading to de novo synthesis of important cytoskeletal regulator proteins. As the first study, we included a proteome analysis of laminin-adherent platelets and interestingly identified upregulation of essentially important proteins that mediate cytoskeletal regulation and mobility in platelets, such as filamin A, talin, vinculin, gelsolin, coronin or kindlin-3. In summary, we demonstrate that platelet activation with extracellular matrix proteins results in a distinct stimulus-specific translational response of platelets that will help to improve our understanding of the regulation of platelet mobility and migration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kraemer, Bjoern F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Geimer, Marc UNSPECIFIED UNSPECIFIED UNSPECIFIED Franz-Wachtel, Mirita UNSPECIFIED UNSPECIFIED UNSPECIFIED Lamkemeyer, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Mannell, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Lindemann, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-313425
DOI:	10.3390/ijms21218155
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	21
Number:	21
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-MIGRATION; TISSUE Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31342