Universität zu Köln

Ehrhardt, Erica ORCID: 0000-0002-9252-1414 and Boyan, George (2020). Evidence for the cholinergic markers ChAT and vAChT in sensory cells of the developing antennal nervous system of the desert locustSchistocerca gregaria. Invertebr. Neurosci., 20 (4). HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1439-1104

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Abstract

Sensory and motor systems in insects with hemimetabolous development must be ready to mediate adaptive behavior directly on hatching from the egg. For the desert locustS. gregaria, cholinergic transmission from antennal sensillae to olfactory or mechanosensory centers in the brain requires that choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (vAChT) already be present in sensory cells in the first instar. In this study, we used immunolabeling to demonstrate that ChAT and vAChT are both expressed in sensory cells from identifiable sensilla types in the immature antennal nervous system. We observed ChAT expression in dendrites, neurites and somata of putative basiconic-type sensillae at the first instar stage. We also detected vAChT in the sensory axons of these sensillae in a major antennal nerve tract. We then examined whether evidence for cholinergic transmission is present during embryogenesis. Immunolabeling confirms that vAChT is expressed in somata typical of campaniform sensillae, as well as in small sensory cell clusters typically associated with either a large basiconic or coeloconic sensilla, at 99% of embryogenesis. The vAChT is also expressed in the somata of these sensilla types in multiple antennal regions at 90% of embryogenesis, but not at earlier (70%) embryonic stages. Neuromodulators are known to appear late in embryogenesis in neurons of the locust central complex, and the cholinergic system of the antenna may also only reach maturity shortly before hatching.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ehrhardt, Erica UNSPECIFIED orcid.org/0000-0002-9252-1414 UNSPECIFIED Boyan, George UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-314448
DOI:	10.1007/s10158-020-00252-4
Journal or Publication Title:	Invertebr. Neurosci.
Volume:	20
Number:	4
Date:	2020
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1439-1104
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VESICULAR ACETYLCHOLINE TRANSPORTER; GAMMA-AMINOBUTYRIC-ACID; EMBRYONIC-DEVELOPMENT; LOCUSTA-MIGRATORIA; PRESYNAPTIC INHIBITION; SCHISTOCERCA-GREGARIA; DROSOPHILA; NEURONS; GRASSHOPPER; RECEPTORS Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31444