Universität zu Köln

Giarola, Valentino ORCID: 0000-0001-6965-4799, Chen, Peilei, Dulitz, Sarah Jane, Koenig, Maurice, Manduzio, Stefano and Bartels, Dorothea (2020). The dehydration- and ABA-inducible germin-like protein CpGLP1 fromCraterostigma plantagineumhas SOD activity and may contribute to cell wall integrity during desiccation. Planta, 252 (5). NEW YORK: SPRINGER. ISSN 1432-2048

Full text not available from this repository.

Abstract

Main conclusion CpGLP1 belongs to the large group of germin-like proteins and comprises a cell wall-localized protein which has superoxide dismutase activity and may contribute towards ROS metabolism and cell wall folding during desiccation. The plant cell wall is a dynamic matrix and its plasticity is essential for cell growth and processing of environmental signals to cope with stresses. A few so-called resurrection plants likeCraterostigma plantagineumsurvive desiccation by implementing protection mechanisms. InC. plantagineum, the cell wall shrinks and folds upon desiccation to avoid mechanical and oxidative damage which contributes to cell integrity. Despite the high toxic potential, ROS are important molecules for cell wall remodeling processes as they participate in enzymatic reactions and act as signaling molecules. Here we analyzed theC. plantagineumgermin-like protein 1 (CpGLP1) to understand its contribution to cell wall folding and desiccation tolerance. The analysis of the CpGLP1 sequence showed that this protein does not fit into the current GLP classification and forms a new group within the Linderniaceae.CpGLP1transcripts accumulate in leaves in response to dehydration and ABA, and mannitol treatments transiently induceCpGLP1transcript accumulation supporting the participation of CpGLP1 in desiccation-related processes. CpGLP1 protein from cell wall protein extracts followed transcript accumulation and protein preparations from bacteria overexpressing CpGLP1 showed SOD activity. In agreement with cell wall localization, CpGLP1 interacts with pectins which have not been reported for GLP proteins. Our data support a role for CpGLP1 in the ROS metabolism related to the control of cell wall plasticity during desiccation inC.plantagineum.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Giarola, Valentino UNSPECIFIED orcid.org/0000-0001-6965-4799 UNSPECIFIED Chen, Peilei UNSPECIFIED UNSPECIFIED UNSPECIFIED Dulitz, Sarah Jane UNSPECIFIED UNSPECIFIED UNSPECIFIED Koenig, Maurice UNSPECIFIED UNSPECIFIED UNSPECIFIED Manduzio, Stefano UNSPECIFIED UNSPECIFIED UNSPECIFIED Bartels, Dorothea UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-315224
DOI:	10.1007/s00425-020-03485-0
Journal or Publication Title:	Planta
Volume:	252
Number:	5
Date:	2020
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-2048
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GLYCINE-RICH PROTEIN; CRATEROSTIGMA-PLANTAGINEUM; SUPEROXIDE-DISMUTASE; GENE FAMILY; OXALATE OXIDASE; PLANT; EXPRESSION; EVOLUTION; REHYDRATION; TOLERANCE Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31522