Universität zu Köln

Hos, Deniz, Hou, Yanhong ORCID: 0000-0003-2248-8441, Witt, Madlen, Barkovskiy, Mikhail, Bock, Felix and Cursiefen, Claus (2020). VEGF Trap(R1R2) Suspended in the Semifluorinated Alkane F6H8 Inhibits Inflammatory Corneal Hem- and Lymphangiogenesis. Transl. Vis. Sci. Technol., 9 (11). ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 2164-2591

Full text not available from this repository.

Abstract

Purpose: Semifluorinated alkanes (SFAs) are used at the ocular surface as lubricants or vehicles for drugs. The purpose of this study was to test the effect of vascular endothelial growth factor (VEGF) Trap(R1R2) suspended in the SFA perfluorohexyloctane (Trap/F6H8) on corneal neovascularization. Methods: Suture placement was used to induce inflammatory corneal neovascularization in mice. Treatment groups were: Trap/F6H8, VEGF Trap(R1R2) as aqueous formulation dissolved in phosphate buffer (Trap), F6H8, and phosphate buffer (controls). Eye drops were applied 3x/daily for 2 weeks. Afterward, corneas were stained with CD31 and LYVE-1 to analyze corneal hem- and lymphangiogenesis. To investigate the effect of on inflammatory cell recruitment, corneal CD45+ cells were quantified. In addition, epithelial wound closure after debridement was assessed by corneal fluorescein staining. Results: Trap/F6H8 was as effective as Trap in inhibiting corneal hemangiogenesis and lymphangiogenesis after 2 weeks of treatment. After 3 days of treatment, Trap/F6H8 was even more effective than Trap in inhibiting corneal hemangiogenesis. Both treatment groups (Trap/F6H8 and Trap) significantly reduced corneal CD45+ cell recruitment. Epithelial closure after debridement was unaffected by Trap/F6H8 or Trap. Conclusions: In this study, we demonstrate that F6H8 is a potential carrier for VEGF Trap(R1R2) to topically treat corneal neovascularization. Our findings might open new treatment avenues for local anti-angiogenic therapy at the cornea, as F6H8 is already approved for the usage at the ocular surface. Translational Relevance: With this study we show for the first time that SFAs can serve as carriers for anti-angiogenic drugs at the ocular surface.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hos, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Hou, Yanhong UNSPECIFIED orcid.org/0000-0003-2248-8441 UNSPECIFIED Witt, Madlen UNSPECIFIED UNSPECIFIED UNSPECIFIED Barkovskiy, Mikhail UNSPECIFIED UNSPECIFIED UNSPECIFIED Bock, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-316192
DOI:	10.1167/tvst.9.11.15
Journal or Publication Title:	Transl. Vis. Sci. Technol.
Volume:	9
Number:	11
Date:	2020
Publisher:	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication:	ROCKVILLE
ISSN:	2164-2591
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DRY EYE DISEASE; TOPICAL RANIBIZUMAB; DROPS; NEOVASCULARIZATION; HEMANGIOGENESIS; ANGIOGENESIS; BEVACIZUMAB; PENETRATION; MECHANISMS; BLOCKADE Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31619