Universität zu Köln

Hulliger, Matthias F. ORCID: 0000-0002-7454-6642, Pacholewska, Alicja ORCID: 0000-0002-4888-4883, Vargas, Amandine, Lavoie, Jean-Pierre, Leeb, Tosso ORCID: 0000-0003-0553-4880, Gerber, Vincent and Jagannathan, Vidhya ORCID: 0000-0002-8155-0041 (2020). An Integrative miRNA-mRNA Expression Analysis Reveals Striking Transcriptomic Similarities between Severe Equine Asthma and Specific Asthma Endotypes in Humans. Genes, 11 (10). BASEL: MDPI. ISSN 2073-4425

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Abstract

Severe equine asthma is an incurable obstructive respiratory condition affecting 10-15% of horses in temperate climates. Upon exposure to airborne antigens from hay feeding, affected horses show neutrophilic airway inflammation and bronchoconstriction, leading to increased respiratory effort. The resulting implications range from welfare concerns to economic impacts on equestrian sports and horse breeding. Immunological and pathophysiological characteristics of severe equine asthma show important parallels with allergic and severe neutrophilic human asthma. Our study aimed at investigating regulatory networks underlying the pathophysiology of the disease by profiling miRNA and mRNA expression in lung tissue samples from asthmatic horses compared with healthy controls. We sequenced small RNAs and mRNAs from lungs of seven asthmatic horses in exacerbation, five affected horses in remission, and eight healthy control horses. Our comprehensive differential expression analyses, combined with the miRNA-mRNA negative correlation approach, revealed a strong similarity on the transcriptomic level between severe equine asthma and severe neutrophilic asthma in humans, potentially through affecting Th17 cell differentiation. This study also showed that several dysregulated miRNAs and mRNAs are involved in airway remodeling. These results present a starting point for a better transcriptomic understanding of severe equine asthma and its similarities to asthma in humans.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hulliger, Matthias F. UNSPECIFIED orcid.org/0000-0002-7454-6642 UNSPECIFIED Pacholewska, Alicja UNSPECIFIED orcid.org/0000-0002-4888-4883 UNSPECIFIED Vargas, Amandine UNSPECIFIED UNSPECIFIED UNSPECIFIED Lavoie, Jean-Pierre UNSPECIFIED UNSPECIFIED UNSPECIFIED Leeb, Tosso UNSPECIFIED orcid.org/0000-0003-0553-4880 UNSPECIFIED Gerber, Vincent UNSPECIFIED UNSPECIFIED UNSPECIFIED Jagannathan, Vidhya UNSPECIFIED orcid.org/0000-0002-8155-0041 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-316329
DOI:	10.3390/genes11101143
Journal or Publication Title:	Genes
Volume:	11
Number:	10
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4425
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RECURRENT AIRWAY-OBSTRUCTION; NF-KAPPA-B; GENE-EXPRESSION; ANIMAL-MODEL; IFN-GAMMA; INFLAMMATION; HORSES; HEAVES; MIR-142-3P; DISEASE Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31632