Koch, Claudia, Kuske, Andra, Joosse, Simon A., Yigit, Goekhan, Sflomos, George ORCID: 0000-0003-2972-0549, Thaler, Sonja, Smit, Daniel J. ORCID: 0000-0002-3190-9511, Werner, Stefan, Borgmann, Kerstin, Gaertner, Sebastian, Mossahebi Mohammadi, Parinaz, Battista, Laura, Cayrefourcq, Laure, Altmueller, Janine, Salinas-Riester, Gabriela, Raithatha, Kaamini, Zibat, Arne, Goy, Yvonne, Ott, Leonie ORCID: 0000-0002-6308-0242, Bartkowiak, Kai, Tan, Tuan Zea, Zhou, Qing, Speicher, Michael R., Mueller, Volkmar, Gorges, Tobias M., Juecker, Manfred, Thiery, Jean-Paul, Brisken, Cathrin, Riethdorf, Sabine, Alix-Panabieres, Catherine and Pantel, Klaus (2020). Characterization of circulating breast cancer cells with tumorigenic and metastatic capacity. EMBO Mol. Med., 12 (9). HOBOKEN: WILEY. ISSN 1757-4684

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Abstract

Functional studies giving insight into the biology of circulating tumor cells (CTCs) remain scarce due to the low frequency ofCTCs and lack of appropriate models. Here, we describe the characterization of a novelCTC-derived breast cancer cell line, designatedCTC-ITB-01, established from a patient with metastatic estrogen receptor-positive (ER+) breast cancer, resistant to endocrine therapy.CTC-ITB-01 remainedER(+)in culture, and copy number alteration (CNA) profiling showed high concordance betweenCTC-ITB-01 andCTCs originally present in the patient with cancer at the time point of blood draw.RNA-sequencing data indicate thatCTC-ITB-01 has a predominantly epithelial expression signature. Primary tumor and metastasis formation in an intraductalPDXmouse model mirrored the clinical progression ofER(+)breast cancer. DownstreamERsignaling was constitutively active inCTC-ITB-01 independent of ligand availability, and theCDK4/6 inhibitor Palbociclib strongly inhibitedCTC-ITB-01 growth. Thus, we established a functional model that opens a new avenue to studyCTCbiology.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Koch, ClaudiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuske, AndraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Joosse, Simon A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yigit, GoekhanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sflomos, GeorgeUNSPECIFIEDorcid.org/0000-0003-2972-0549UNSPECIFIED
Thaler, SonjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Smit, Daniel J.UNSPECIFIEDorcid.org/0000-0002-3190-9511UNSPECIFIED
Werner, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Borgmann, KerstinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gaertner, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mossahebi Mohammadi, ParinazUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Battista, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cayrefourcq, LaureUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Altmueller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Salinas-Riester, GabrielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Raithatha, KaaminiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zibat, ArneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Goy, YvonneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ott, LeonieUNSPECIFIEDorcid.org/0000-0002-6308-0242UNSPECIFIED
Bartkowiak, KaiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tan, Tuan ZeaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhou, QingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Speicher, Michael R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mueller, VolkmarUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gorges, Tobias M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Juecker, ManfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thiery, Jean-PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brisken, CathrinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Riethdorf, SabineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Alix-Panabieres, CatherineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pantel, KlausUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-319610
DOI: 10.15252/emmm.201911908
Journal or Publication Title: EMBO Mol. Med.
Volume: 12
Number: 9
Date: 2020
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1757-4684
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EPITHELIAL-MESENCHYMAL TRANSITION; KINASE 4/6 INHIBITOR; TUMOR-CELLS; STEM-CELLS; EXPRESSION; MUTATIONS; PALBOCICLIB; SURVIVAL; IDENTIFICATION; CHALLENGESMultiple languages
Medicine, Research & ExperimentalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/31961

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