Universität zu Köln

Gerbracht, Jennifer, V, Boehm, Volker, Britto-Borges, Thiago, Kallabis, Sebastian, Wiederstein, Janica L., Ciriello, Simona, Aschemeier, Dominik U., Krueger, Marcus, Frese, Christian K., Altmueller, Janine, Dieterich, Christoph and Gehring, Niels H. ORCID: 0000-0001-7792-1164 (2020). CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complex. Nucleic Acids Res., 48 (15). S. 8626 - 8645. OXFORD: OXFORD UNIV PRESS. ISSN 1362-4962

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Abstract

The exon junction complex (EJC) is an essential constituent and regulator of spliced messenger ribonucleoprotein particles (mRNPs) in metazoans. As a core component of the EJC, CASC3 was described to be pivotal for EJC-dependent nuclear and cytoplasmic processes. However, recent evidence suggests that CASC3 functions differently from other EJC core proteins. Here, we have established human CASC3 knockout cell lines to elucidate the cellular role of CASC3. In the knockout cells, overall EJC composition and EJC-dependent splicing are unchanged. A transcriptome-wide analysis reveals that hundreds of mRNA isoforms targeted by nonsense-mediated decay (NMD) are upregulated. Mechanistically, recruiting CASC3 to reporter mRNAs by direct tethering or via binding to the EJC stimulates mRNA decay and endonucleolytic cleavage at the termination codon. Building on existing EJC-NMD models, we propose that CASC3 equips the EJC with the persisting ability to communicate with the NMD machinery in the cytoplasm. Collectively, our results characterize CASC3 as a peripheral EJC protein that tailors the transcriptome by promoting the degradation of EJC-dependent NMD substrates.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gerbracht, Jennifer, V UNSPECIFIED UNSPECIFIED UNSPECIFIED Boehm, Volker UNSPECIFIED UNSPECIFIED UNSPECIFIED Britto-Borges, Thiago UNSPECIFIED UNSPECIFIED UNSPECIFIED Kallabis, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiederstein, Janica L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ciriello, Simona UNSPECIFIED UNSPECIFIED UNSPECIFIED Aschemeier, Dominik U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED Frese, Christian K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Dieterich, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehring, Niels H. UNSPECIFIED orcid.org/0000-0001-7792-1164 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-319719
DOI:	10.1093/nar/gkaa564
Journal or Publication Title:	Nucleic Acids Res.
Volume:	48
Number:	15
Page Range:	S. 8626 - 8645
Date:	2020
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1362-4962
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESSENGER-RNA DECAY; CRYSTAL-STRUCTURE; CORE COMPLEX; PROTEIN; BINDING; NMD; QUANTIFICATION; IDENTIFICATION; TRANSLATION; EIF4AIII Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/31971