Universität zu Köln

Sawa-Makarska, Justyna, Baumann, Verena, Coudevylle, Nicolas, von Bulow, Soren ORCID: 0000-0001-7125-5973, Nogellova, Veronika, Abert, Christine, Schuschnig, Martina, Graef, Martin, Hummer, Gerhard ORCID: 0000-0001-7768-746X and Martens, Sascha ORCID: 0000-0003-3786-8199 (2020). Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation. Science, 369 (6508). S. 1206 - 1265. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

Full text not available from this repository.

Abstract

Autophagosomes form de novo in a manner that is incompletely understood. Particularly enigmatic are autophagy-related protein 9 (Atg9)-containing vesicles that are required for autophagy machinery assembly but do not supply the bulk of the autophagosomal membrane. In this study, we reconstituted autophagosome nucleation using recombinant components from yeast. We found that Atg9 proteoliposomes first recruited the phosphatidylinositol 3-phosphate kinase complex, followed by Atg21, the Atg2-Atg18 lipid transfer complex, and the E3-like Atg12-Atg5-Atg16 complex, which promoted Atg8 lipidation. Furthermore, we found that Atg2 could transfer lipids for Atg8 lipidation. In selective autophagy, these reactions could potentially be coupled to the cargo via the Atg19-Atg11-Atg9 interactions. We thus propose that Atg9 vesicles form seeds that establish membrane contact sites to initiate lipid transfer from compartments such as the endoplasmic reticulum.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sawa-Makarska, Justyna UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumann, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Coudevylle, Nicolas UNSPECIFIED UNSPECIFIED UNSPECIFIED von Bulow, Soren UNSPECIFIED orcid.org/0000-0001-7125-5973 UNSPECIFIED Nogellova, Veronika UNSPECIFIED UNSPECIFIED UNSPECIFIED Abert, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuschnig, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Graef, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hummer, Gerhard UNSPECIFIED orcid.org/0000-0001-7768-746X UNSPECIFIED Martens, Sascha UNSPECIFIED orcid.org/0000-0003-3786-8199 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-319765
DOI:	10.1126/science.aaz7714
Journal or Publication Title:	Science
Volume:	369
Number:	6508
Page Range:	S. 1206 - 1265
Date:	2020
Publisher:	AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication:	WASHINGTON
ISSN:	1095-9203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SELECTIVE AUTOPHAGY; MOLECULAR-DYNAMICS; PHOSPHATIDYLINOSITOL 3-PHOSPHATE; ENDOPLASMIC-RETICULUM; ATG12-ATG5 CONJUGATE; PROTEIN-STRUCTURE; BINDING-SITES; COMPLEX; YEAST; BIOGENESIS Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31976