Universität zu Köln

Terashima, Ryota ORCID: 0000-0002-9339-6651, Saigo, Tomotaka, Laoharatchatathanin, Titaree, Kurusu, Shiro, Brachvogel, Bent, Poeschl, Ernst and Kawaminami, Mitsumori ORCID: 0000-0003-3165-4413 (2020). Augmentation of Nr4a3 and Suppression of Fshb Expression in the Pituitary Gland of Female Annexin A5 Null Mouse. J. Endocr. Soc., 4 (9). WASHINGTON: ENDOCRINE SOC. ISSN 2472-1972

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Abstract

GnRH enhances the expression of annexin A5 (ANXA5) in pituitary gonadotropes, and ANXA5 enhances gonadotropin secretion. However, the impact of ANXA5 regulation on the expression of pituitary hormone genes remains unclear. Here, using quantitative PCR, we demonstrated that ANXA5 deficiency in female mice reduced the expression of Fshb and Gh in their pituitary glands. Transcriptome analysis confirmed a specific increase in Nr4a3 mRNA expression in addition to lower levels of Fshb expression in ANXA5-deficient female pituitary glands. This gene was then found to be a GnRH-inducible immediate early gene, and its increased expression caused protein to accumulate in the nucleus after administration of a GnRH agonist in L beta T2 cells, which are an in vitro pituitary gonadotrope model. The increase in ANXA5 protein levels in L beta T2 cells clearly suppressed Nr4a3 expression. siRNA-mediated inhibition of Nr4a3 expression increased Fshb expression. The results revealed that GnRH stimulates Nr4a3 and Anxa5 sequentially. NR4A3 suppression of Fshb may be necessary for later massive secretion of FSH by GnRH in gonadotropes, and Nr4a3 would be negatively regulated by ANXA5 to increase FSH secretion. (C) Endocrine Society 2020.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Terashima, Ryota UNSPECIFIED orcid.org/0000-0002-9339-6651 UNSPECIFIED Saigo, Tomotaka UNSPECIFIED UNSPECIFIED UNSPECIFIED Laoharatchatathanin, Titaree UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurusu, Shiro UNSPECIFIED UNSPECIFIED UNSPECIFIED Brachvogel, Bent UNSPECIFIED UNSPECIFIED UNSPECIFIED Poeschl, Ernst UNSPECIFIED UNSPECIFIED UNSPECIFIED Kawaminami, Mitsumori UNSPECIFIED orcid.org/0000-0003-3165-4413 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-320489
DOI:	10.1210/jendso/bvaa096
Journal or Publication Title:	J. Endocr. Soc.
Volume:	4
Number:	9
Date:	2020
Publisher:	ENDOCRINE SOC
Place of Publication:	WASHINGTON
ISSN:	2472-1972
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GONADOTROPIN-RELEASING-HORMONE; ORPHAN NUCLEAR RECEPTOR; GENE-EXPRESSION; DEPENDENT REGULATION; BETA-GENE; FREQUENCY; NOR-1; SECRETION; CELLS; GNRH Multiple languages Endocrinology & Metabolism Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32048