Universität zu Köln

Kim, Sang-Jin ORCID: 0000-0002-7186-6202, Chandrasekar, Balakumaran, Rea, Anne C., Danhof, Linda, Zemelis-Durfee, Starla, Thrower, Nicholas ORCID: 0000-0002-7357-6639, Shepard, Zachary S., Pauly, Markus ORCID: 0000-0002-3116-2198, Brandizzi, Federica and Keegstra, Kenneth (2020). The synthesis of xyloglucan, an abundant plant cell wall polysaccharide, requires CSLC function. Proc. Natl. Acad. Sci. U. S. A., 117 (33). S. 20316 - 20325. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Xyloglucan (XyG) is an abundant component of the primary cell walls of most plants. While the structure of XyG has been well studied, much remains to be learned about its biosynthesis. Here we employed reverse genetics to investigate the role of Arabidopsis cellulose synthase like-C (CSLC) proteins in XyG biosynthesis. We found that single mutants containing a T-DNA in each of the five Arabidopsis CSLC genes had normal levels of XyG. However, higher-order cslc mutants had significantly reduced XyG levels, and a mutant with disruptions in all five CSLC genes had no detectable XyG. The higher-order mutants grew with mild tissuespecific phenotypes. Despite the apparent lack of XyG, the cslc quintuple mutant did not display significant alteration of gene expression at the whole-genome level, excluding transcriptional compensation. The quintuple mutant could be complemented by each of the five CSLC genes, supporting the conclusion that each of them encodes a XyG glucan synthase. Phylogenetic analyses indicated that the CSLC genes are widespread in the plant kingdom and evolved from an ancient family. These results establish the role of the CSLC genes in XyG biosynthesis, and the mutants described here provide valuable tools with which to study both the molecular details of XyG biosynthesis and the role of XyG in plant cell wall structure and function.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kim, Sang-Jin UNSPECIFIED orcid.org/0000-0002-7186-6202 UNSPECIFIED Chandrasekar, Balakumaran UNSPECIFIED UNSPECIFIED UNSPECIFIED Rea, Anne C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Danhof, Linda UNSPECIFIED UNSPECIFIED UNSPECIFIED Zemelis-Durfee, Starla UNSPECIFIED UNSPECIFIED UNSPECIFIED Thrower, Nicholas UNSPECIFIED orcid.org/0000-0002-7357-6639 UNSPECIFIED Shepard, Zachary S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pauly, Markus UNSPECIFIED orcid.org/0000-0002-3116-2198 UNSPECIFIED Brandizzi, Federica UNSPECIFIED UNSPECIFIED UNSPECIFIED Keegstra, Kenneth UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-323047
DOI:	10.1073/pnas.2007245117
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	117
Number:	33
Page Range:	S. 20316 - 20325
Date:	2020
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELLULOSE-SYNTHASE-LIKE; FAMILY-MEMBERS; SEQ ANALYSIS; ARABIDOPSIS; GENE; GALACTOSYLTRANSFERASE; IDENTIFICATION; BIOSYNTHESIS; DEFICIENT; EVOLUTION Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32304