Universität zu Köln

Mathew, Renjith, Rios-Barrera, L. Daniel, Machado, Pedro, Schwab, Yannick ORCID: 0000-0001-8027-1836 and Leptin, Maria ORCID: 0000-0001-7097-348X (2020). Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism. Embo J., 39 (16). HOBOKEN: WILEY. ISSN 1460-2075

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Abstract

Plasma membranes fulfil many physiological functions. In polarized cells, different membrane compartments take on specialized roles, each being allocated correct amounts of membrane. TheDrosophilatracheal system, an established tubulogenesis model, contains branched terminal cells with subcellular tubes formed by apical plasma membrane invagination. We show that apical endocytosis and late endosome-mediated trafficking are required for membrane allocation to the apical and basal membrane domains. Basal plasma membrane growth stops if endocytosis is blocked, whereas the apical membrane grows excessively. Plasma membrane is initially delivered apically and then continuously endocytosed, together with apical and basal cargo. We describe an organelle carrying markers of late endosomes and multivesicular bodies (MVBs) that is abolished by inhibiting endocytosis and which we suggest acts as transit station for membrane destined to be redistributed both apically and basally. This is based on the observation that disrupting MVB formation prevents growth of both compartments.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mathew, Renjith UNSPECIFIED UNSPECIFIED UNSPECIFIED Rios-Barrera, L. Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Machado, Pedro UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwab, Yannick UNSPECIFIED orcid.org/0000-0001-8027-1836 UNSPECIFIED Leptin, Maria UNSPECIFIED orcid.org/0000-0001-7097-348X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-323119
DOI:	10.15252/embj.2020105332
Journal or Publication Title:	Embo J.
Volume:	39
Number:	16
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1460-2075
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BRANCHING MORPHOGENESIS; MEMBRANE TRAFFICKING; TRACHEAL LUMEN; APICAL SURFACE; DROSOPHILA; PROTEIN; SECRETION; CRUMBS; MATURATION; TRANSPORT Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/32311