Universität zu Köln

Baeza, Luis Lucena and Hamprecht, Axel ORCID: 0000-0003-1449-5780 (2020). A profile of the GenePOC Carba C assay for the detection and differentiation of gene sequences associated with carbapenem-non-susceptibility. Expert Rev. Mol. Diagn., 20 (8). S. 757 - 770. OSLO: TAYLOR & FRANCIS AS. ISSN 1744-8352

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Abstract

The novel GenePOC/Revogene Carba C assay (GenePOC, Quebec, Canada; now Meridian Bioscience, Cincinnati, OH, USA) is a CE-IVD marked, FDA-approved qualitative in vitro diagnostic test for the detection of genes associated with carbapenem-non-susceptibility. Colonies of Enterobacterales can be directly tested without prior DNA isolation. The test consists of a fluorescent-based real-time PCR assay that runs on the centripetal microfluidic revogene platform, providing results within 70 minutes. The assay was evaluated in two studies comprising a total of 294 molecularly characterized clinical Enterobacterales isolates. The overall sensitivity for the detection of carbapenemase gene sequences with the GenePOC assay was 100% (95% CI, 98.4% to 100). Besides the common KPC, VIM, NDM and OXA-48-like carbapenemase genes, also the very variable IMP variants were all detected. The specificity of the assay was 100% (95% CI, 98.8% to 100%). In this article the performance of the GenePOC/ Revogene Carba C assay is evaluated and other currently available methods for the detection of carbapenemases are reviewed.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Baeza, Luis Lucena UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-326963
DOI:	10.1080/14737159.2020.1785287
Journal or Publication Title:	Expert Rev. Mol. Diagn.
Volume:	20
Number:	8
Page Range:	S. 757 - 770
Date:	2020
Publisher:	TAYLOR & FRANCIS AS
Place of Publication:	OSLO
ISSN:	1744-8352
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; CHECK-DIRECT CPE; RAPID DETECTION; LABORATORY DETECTION; BETA-LACTAMASES; MULTIPLEX PCR; BLUE-CARBA; KLEBSIELLA-PNEUMONIAE; NP TEST Multiple languages Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32696