Universität zu Köln

Zeitler, Leo ORCID: 0000-0002-4302-1739, Ross-Ibarra, Jeffrey ORCID: 0000-0003-1656-4954 and Stetter, Markus G. ORCID: 0000-0001-7136-0589 (2020). Selective Loss of Diversity in Doubled-Haploid Lines from European Maize Landraces. G3-Genes Genomes Genet., 10 (7). S. 2497 - 2507. BETHESDA: GENETICS SOCIETY AMERICA. ISSN 2160-1836

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Abstract

Maize landraces are well adapted to their local environments and present valuable sources of genetic diversity for breeding and conservation. But the maintenance of open-pollinated landraces inex-situprograms is challenging, as regeneration of seed can often lead to inbreeding depression and the loss of diversity due to genetic drift. Recent reports suggest that the production of doubled-haploid (DH) lines from landraces may serve as a convenient means to preserve genetic diversity in a homozygous form that is immediately useful for modern breeding. The production of doubled-haploid (DH) lines presents an extreme case of inbreeding which results in instantaneous homozygosity genome-wide. Here, we analyzed the effect of DH production on genetic diversity, using genome-wide SNP data from hundreds of individuals of five European landraces and their related DH lines. In contrast to previous findings, we observe a dramatic loss of diversity at both the haplotype level and that of individual SNPs. We identify thousands of SNPs that exhibit allele frequency differences larger than expected under models of neutral genetic drift and document losses of shared haplotypes. We find evidence consistent with selection at functional sites that are potentially involved in the diversity differences between landrace and DH populations. Although we were unable to uncover more details about the mode of selection, we conclude that landrace DH lines may be a valuable tool for the introduction of variation into maize breeding programs but come at the cost of decreased genetic diversity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zeitler, Leo UNSPECIFIED orcid.org/0000-0002-4302-1739 UNSPECIFIED Ross-Ibarra, Jeffrey UNSPECIFIED orcid.org/0000-0003-1656-4954 UNSPECIFIED Stetter, Markus G. UNSPECIFIED orcid.org/0000-0001-7136-0589 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-327668
DOI:	10.1534/g3.120.401196
Journal or Publication Title:	G3-Genes Genomes Genet.
Volume:	10
Number:	7
Page Range:	S. 2497 - 2507
Date:	2020
Publisher:	GENETICS SOCIETY AMERICA
Place of Publication:	BETHESDA
ISSN:	2160-1836
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DELETERIOUS MUTATIONS; GENETIC DIVERSITY; GENOME; ADAPTATION; SIZE; TOOL Multiple languages Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/32766