Universität zu Köln

Pham, Vinh Ngoc ORCID: 0000-0001-6802-5827, Paik, Inyup, Hoecker, Ute ORCID: 0000-0002-5636-9777 and Huq, Enamul ORCID: 0000-0001-7692-5139 (2020). Genomic evidence revealsSPA-regulated developmental and metabolic pathways in dark-grownArabidopsisseedlings. Physiol. Plant., 169 (3). S. 380 - 397. HOBOKEN: WILEY. ISSN 1399-3054

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Abstract

Photomorphogenesis is repressed in the dark mainly by an E3 ubiquitin ligase complex comprising CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and four homologous proteins called SUPPRESSOR OF PHYA-105 (SPA1-SPA4) in Arabidopsis. This complex induces the ubiquitination and subsequent degradation of positively acting transcription factors (TFs; e.g. ELONGATED HYPOCOTYL (HY5), LONG HYPOCOTYL IN FAR-RED 1 (HFR1), PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) and others] in the dark to repress photomorphogenesis. Genomic evidence showed a large number of genes regulated by COP1 in the dark, of which many are direct targets of HY5. However, the genomic basis for the constitute photomorphogenic phenotype ofspaQremains unknown. Here, we show that >7200 genes are differentially expressed in thespaQbackground compared to wild-type in the dark. Comparison of the RNA sequencing (RNA-Seq) data betweencop1andspaQrevealed a large overlapping set of genes regulated by the COP1-SPA complex. In addition, many of the genes coordinately regulated by the COP1-SPA complex are also regulated by HY5 directly and indirectly. Taken together, our data reveal that SPA proteins repress photomorphogenesis by controlling gene expression in concert with COP1, likely through regulating the abundance of downstream TFs in light signaling pathways. Moreover, SPA proteins may function both in a COP1-dependent and -independent manner in regulating many biological processes and developmental pathways in Arabidopsis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pham, Vinh Ngoc UNSPECIFIED orcid.org/0000-0001-6802-5827 UNSPECIFIED Paik, Inyup UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED orcid.org/0000-0002-5636-9777 UNSPECIFIED Huq, Enamul UNSPECIFIED orcid.org/0000-0001-7692-5139 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-328211
DOI:	10.1111/ppl.13095
Journal or Publication Title:	Physiol. Plant.
Volume:	169
Number:	3
Page Range:	S. 380 - 397
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1399-3054
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language E3 UBIQUITIN LIGASE; GENE-EXPRESSION; LIGHT CONTROL; SEEDLING PHOTOMORPHOGENESIS; REPRESS PHOTOMORPHOGENESIS; MEDIATED REGULATION; COP9 SIGNALOSOME; ARABIDOPSIS; PHYTOCHROME; PROTEINS Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/32821