Erger, Florian ORCID: 0000-0002-2768-1702, Noerling, Deborah, Borchert, Domenica, Leenen, Esther, Habbig, Sandra, Wiesener, Michael S., Bartram, Malte P., Wenzel, Andrea, Becker, Christian, Toliat, Mohammad R., Nuernberg, Peter, Beck, Bodo B. and Altmueller, Janine (2020). cfNOMe- A single assay for comprehensive epigenetic analyses of cell-free DNA. Genome Med., 12 (1). LONDON: BMC. ISSN 1756-994X

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Abstract

Cell-free DNA (cfDNA) analysis has become essential in cancer diagnostics and prenatal testing. We presentcfNOMe, a two-in-one method of measuring cfDNA cytosine methylation and nucleosome occupancy in a single assay using non-disruptive enzymatic cytosine conversion and a custom bioinformatic pipeline. We show that enzymatic cytosine conversion better preserves cfDNA fragmentation information than does bisulfite conversion. Whereas previously separate experiments were required to study either epigenetic marking,cfNOMedelivers reliable results for both, enabling more comprehensive and inexpensive epigenetic cfDNA profiling.cfNOMehas the potential to advance biomarker discovery and diagnostic usage in diseases with systemic perturbations of cfDNA composition.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Erger, FlorianUNSPECIFIEDorcid.org/0000-0002-2768-1702UNSPECIFIED
Noerling, DeborahUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Borchert, DomenicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leenen, EstherUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Habbig, SandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wiesener, Michael S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bartram, Malte P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wenzel, AndreaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Becker, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Toliat, Mohammad R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nuernberg, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Beck, Bodo B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Altmueller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-329226
DOI: 10.1186/s13073-020-00750-5
Journal or Publication Title: Genome Med.
Volume: 12
Number: 1
Date: 2020
Publisher: BMC
Place of Publication: LONDON
ISSN: 1756-994X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
METHYLATIONMultiple languages
Genetics & HeredityMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/32922

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