Universität zu Köln

Solheim, Sara Amalie ORCID: 0000-0002-4589-1711, Jessen, Soren ORCID: 0000-0002-7461-1264, Morkeberg, Jakob, Thevis, Mario, Dehnes, Yvette, Eibye, Kasper ORCID: 0000-0002-5737-691X, Hostrup, Morten ORCID: 0000-0002-6201-2483 and Nordsborg, Nikolai Baastrup (2020). Single-dose administration of clenbuterol is detectable in dried blood spots. Drug Test. Anal., 12 (9). S. 1366 - 1373. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

Clenbuterol is a beta(2)-agonist prescribed for asthmatic patients in some countries. Based on its anabolic and lipolytic effects observed in studies on rodents and in livestock destined for food production, clenbuterol is abused by bodybuilders and athletes seeking leanness. Urinary clenbuterol analysis is part of routine doping analysis. However, the collection of urine samples is time-consuming and can be intimidating for athletes. Dried blood spot (DBS) appears attractive as an alternative matrix, but the detectability of clenbuterol in humans through DBS has not been investigated. This study evaluated if clenbuterol could be detected in DBS and urine collected from six healthy men after oral intake of 80 mu g clenbuterol. The DBS and urine samples were collected at 0, 3, 8, 24, and 72 h post-ingestion, with additional urine collections on days 7 and 10. Using LC-MS/MS, it was shown that clenbuterol could be detected in all DBS samples for 24 h post-ingestion and with 50% sensitivity 3 days after ingestion. The DBS method was 100% specific. Evaluation of analyte stability showed that clenbuterol is stable in DBS for at least 365 days at room temperature when using desiccant and avoiding light exposure. In urine, clenbuterol was detectable for at least 7-10 days after ingestion. Urinary clenbuterol concentrations below 5 ng/mL were present in some subjects 24 h after administration. Collectively, these data indicate that DBS are suitable for routine doping control analysis of clenbuterol with a detection window of at least 3 days after oral administration of 80 mu g.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Solheim, Sara Amalie UNSPECIFIED orcid.org/0000-0002-4589-1711 UNSPECIFIED Jessen, Soren UNSPECIFIED orcid.org/0000-0002-7461-1264 UNSPECIFIED Morkeberg, Jakob UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Dehnes, Yvette UNSPECIFIED UNSPECIFIED UNSPECIFIED Eibye, Kasper UNSPECIFIED orcid.org/0000-0002-5737-691X UNSPECIFIED Hostrup, Morten UNSPECIFIED orcid.org/0000-0002-6201-2483 UNSPECIFIED Nordsborg, Nikolai Baastrup UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-329464
DOI:	10.1002/dta.2872
Journal or Publication Title:	Drug Test. Anal.
Volume:	12
Number:	9
Page Range:	S. 1366 - 1373
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DOPING CONTROL ANALYSIS; FOOD CONTAMINATION; MASS; QUANTIFICATION; MUSCLE; URINE; PHARMACOKINETICS; HYPERTROPHY; ABUSE; RAT Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32946