Universität zu Köln

Schubert, Marcel ORCID: 0000-0002-8739-4852, Woolfson, Lewis, Barnard, Isla R. M., Dorward, Amy M., Casement, Becky, Morton, Andrew, Robertson, Gavin B., Appleton, Paul L., Miles, Gareth B., Tucker, Carl S., Pitt, Samantha J. and Gather, Malte C. ORCID: 0000-0002-4857-5562 (2020). Monitoring contractility in cardiac tissue with cellular resolution using biointegrated microlasers. Nat. Photonics, 14 (7). S. 452 - 464. LONDON: NATURE PUBLISHING GROUP. ISSN 1749-4893

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Abstract

The incorporation of microdisk lasers into heart cells allows all-optical recording of cardiac contraction with cellular resolution. The contractility of cardiac cells is a key parameter that describes the biomechanical characteristics of the beating heart, but functional monitoring of three-dimensional cardiac tissue with single-cell resolution remains a major challenge. Here, we introduce microscopic whispering-gallery-mode lasers into cardiac cells to realize all-optical recording of transient cardiac contraction profiles with cellular resolution. The brilliant emission and high spectral sensitivity of microlasers to local changes in refractive index enable long-term tracking of individual cardiac cells, monitoring of drug administration, accurate measurements of organ-scale contractility in live zebrafish, and robust contractility sensing through hundreds of micrometres of rat heart tissue. Our study reveals changes in sarcomeric protein density as an underlying factor to cardiac contraction. More broadly, the use of novel micro- and nanoscopic lasers as non-invasive, biointegrated optical sensors brings new opportunities to monitor a wide range of physiological parameters with cellular resolution.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schubert, Marcel UNSPECIFIED orcid.org/0000-0002-8739-4852 UNSPECIFIED Woolfson, Lewis UNSPECIFIED UNSPECIFIED UNSPECIFIED Barnard, Isla R. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dorward, Amy M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Casement, Becky UNSPECIFIED UNSPECIFIED UNSPECIFIED Morton, Andrew UNSPECIFIED UNSPECIFIED UNSPECIFIED Robertson, Gavin B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Appleton, Paul L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Miles, Gareth B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tucker, Carl S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pitt, Samantha J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gather, Malte C. UNSPECIFIED orcid.org/0000-0002-4857-5562 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-329970
DOI:	10.1038/s41566-020-0631-z
Journal or Publication Title:	Nat. Photonics
Volume:	14
Number:	7
Page Range:	S. 452 - 464
Date:	2020
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	1749-4893
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Physical Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SARCOMERE-LENGTH; CELLS Multiple languages Optics; Physics, Applied Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/32997