Universität zu Köln

Walter, Sebastian G., Randau, Thomas M., Hilgers, Caecilia, Haddouti, El-Mustapha, Masson, Werner, Gravius, Sascha, Burger, Christof, Wirtz, Dieter C. and Schildberg, Frank A. (2020). Molecular and Functional Phenotypes of Human Bone Marrow-Derived Mesenchymal Stromal Cells Depend on Harvesting Techniques. Int. J. Mol. Sci., 21 (12). BASEL: MDPI. ISSN 1422-0067

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Abstract

Mesenchymal stromal cells (MSC) harvested in different tissues from the same donor exhibit different phenotypes. Each phenotype is not only characterized by a certain pattern of cell surface markers, but also different cellular functionalities. Only recently were different harvesting and processing techniques found to contribute to this phenomenon as well. This study was therefore set up to investigate proteomic and functional properties of human bone marrow-derived MSCs (hBM-MSC). These were taken from the same tissue and donor site but harvested either as aspirate or bone chip cultures. Both MSC populations were profiled for MSC markers defined by the International Society for Cellular Therapy (ISCT), MSC markers currently under discussion and markers of particular interest. While classic ISCT MSC markers did not show any significant difference between aspirate and outgrowth hBM-MSCs, our additional characterization panel revealed distinct patterns of differentially expressed markers. Furthermore, hBM-MSCs from aspirate cultures demonstrated a significantly higher osteogenic differentiation potential than outgrowth MSCs, which could be confirmed using a transcriptional approach. Our comparison of MSC phenotypes obtained by different harvesting techniques suggests the need of future standardized harvesting, processing and phenotyping procedures in order to gain better comparability in the MSC field.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Walter, Sebastian G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Randau, Thomas M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hilgers, Caecilia UNSPECIFIED UNSPECIFIED UNSPECIFIED Haddouti, El-Mustapha UNSPECIFIED UNSPECIFIED UNSPECIFIED Masson, Werner UNSPECIFIED UNSPECIFIED UNSPECIFIED Gravius, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED Burger, Christof UNSPECIFIED UNSPECIFIED UNSPECIFIED Wirtz, Dieter C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildberg, Frank A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-331279
DOI:	10.3390/ijms21124382
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	21
Number:	12
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STEM-CELLS; MARKERS; MSC Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33127