Universität zu Köln

Goudet, Myriam M. M., Orr, Douglas J. ORCID: 0000-0003-1217-537X, Melkonian, Michael, Muller, Karin H., Meyer, Moritz T., Carmo-Silva, Elizabete ORCID: 0000-0001-6059-9359 and Griffiths, Howard (2020). Rubisco and carbon-concentrating mechanism co-evolution across chlorophyte and streptophyte green algae. New Phytol., 227 (3). S. 810 - 824. HOBOKEN: WILEY. ISSN 1469-8137

Full text not available from this repository.

Abstract

Green algae expressing a carbon-concentrating mechanism (CCM) are usually associated with a Rubisco-containing micro-compartment, the pyrenoid. A link between the small subunit (SSU) of Rubisco and pyrenoid formation in Chlamydomonas reinhardtii has previously suggested that specific RbcS residues could explain pyrenoid occurrence in green algae. A phylogeny of RbcS was used to compare the protein sequence and CCM distribution across the green algae and positive selection in RbcS was estimated. For six streptophyte algae, Rubisco catalytic properties, affinity for CO2 uptake (K-0.5), carbon isotope discrimination (delta C-13) and pyrenoid morphology were compared. The length of the beta A-beta B loop in RbcS provided a phylogenetic marker discriminating chlorophyte from streptophyte green algae. Rubisco kinetic properties in streptophyte algae have responded to the extent of inducible CCM activity, as indicated by changes in inorganic carbon uptake affinity, delta C-13 and pyrenoid ultrastructure between high and low CO2 conditions for growth. We conclude that the Rubisco catalytic properties found in streptophyte algae have coevolved and reflect the strength of any CCM or degree of pyrenoid leakiness, and limitations to inorganic carbon in the aquatic habitat, whereas Rubisco in extant land plants reflects more recent selective pressures associated with improved diffusive supply of the terrestrial environment.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goudet, Myriam M. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Orr, Douglas J. UNSPECIFIED orcid.org/0000-0003-1217-537X UNSPECIFIED Melkonian, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Muller, Karin H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Moritz T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Carmo-Silva, Elizabete UNSPECIFIED orcid.org/0000-0001-6059-9359 UNSPECIFIED Griffiths, Howard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-333899
DOI:	10.1111/nph.16577
Journal or Publication Title:	New Phytol.
Volume:	227
Number:	3
Page Range:	S. 810 - 824
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SMALL-SUBUNIT; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE; CO2-CONCENTRATING MECHANISMS; CHLAMYDOMONAS-REINHARDTII; TEMPERATURE RESPONSES; PHYLOGENETIC ANALYSIS; PYRENOID MORPHOLOGY; PROTEIN; REVEALS; CO2 Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33389