Universität zu Köln

Demir, Muenevver, Lang, Sonja, Martin, Anna, Farowski, Fedja, Wisplinghoff, Hilmar, Vehreschild, Maria J. G. T., Krawczyk, Marcin ORCID: 0000-0002-0113-0777, Nowag, Angela, Scholz, Claus Jurgen, Kretzschmar, Anne, Roderburg, Christoph, Lammert, Frank, Goeser, Tobias, Kasper, Philipp and Steffen, Hans-Michael (2020). Phenotyping non-alcoholic fatty liver disease by the gut microbiota: Ready for prime time? J. Gastroenterol. Hepatol., 35 (11). S. 1969 - 1978. HOBOKEN: WILEY. ISSN 1440-1746

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Abstract

Background and Aim Several studies observed alterations in the gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD). However, analyzed patient populations and methods strongly differ among these studies. The aim of this study was to prove the reproducibility of published results and to provide a detailed overview of all findings in our NAFLD cohort using next generation sequencing methods. Methods The individual taxonomic microbiota composition of fecal samples from 90 NAFLD patients and 21 healthy controls was analyzed using 16S rRNA gene sequencing. Study participants were grouped according to their disease stage and compared regarding their gut microbiota composition. Studies were identified from PubMed listed publications, and the results were compared with the findings in our cohort. Results Results from 13 identified studies were compared with our data. A decreased abundance of the Bacteroidetes and Ruminococcaceae as well as an increased abundance of Lactobacillaceae and Veillonellaceae and Dorea were the most frequently reported changes among NAFLD patients in 4/13, 5/13, 4/13, 2/13, and 3/13 studies, respectively. Even though these alterations in the gut microbiota composition were also observed in our patient cohort, the majority of published differences could not be reproduced, neither in our own nor in other NAFLD cohort studies. Conclusion Despite repeatedly reproduced abundance patterns of specific bacteria, the heterogeneous study results did not reveal a consistent disease specific gut microbiota signature. Further prospective studies with homogenous patient cohorts and standardized methods are necessary to phenotype NAFLD by the gut microbiota.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Demir, Muenevver UNSPECIFIED UNSPECIFIED UNSPECIFIED Lang, Sonja UNSPECIFIED UNSPECIFIED UNSPECIFIED Martin, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Farowski, Fedja UNSPECIFIED UNSPECIFIED UNSPECIFIED Wisplinghoff, Hilmar UNSPECIFIED UNSPECIFIED UNSPECIFIED Vehreschild, Maria J. G. T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Krawczyk, Marcin UNSPECIFIED orcid.org/0000-0002-0113-0777 UNSPECIFIED Nowag, Angela UNSPECIFIED UNSPECIFIED UNSPECIFIED Scholz, Claus Jurgen UNSPECIFIED UNSPECIFIED UNSPECIFIED Kretzschmar, Anne UNSPECIFIED UNSPECIFIED UNSPECIFIED Roderburg, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Lammert, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Goeser, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Kasper, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Steffen, Hans-Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-336185
DOI:	10.1111/jgh.15071
Journal or Publication Title:	J. Gastroenterol. Hepatol.
Volume:	35
Number:	11
Page Range:	S. 1969 - 1978
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1440-1746
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTESTINAL MICROBIOTA; METABOLIC SYNDROME; STEATOHEPATITIS; DYSBIOSIS; FIBROSIS; OBESITY Multiple languages Gastroenterology & Hepatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33618