Universität zu Köln

Sahu, Preeti ORCID: 0000-0003-1741-1642, Sussman, Daniel M., Ruebsam, Matthias, Mertz, Aaron F., Horsley, Valerie, Dufresne, Eric R., Niessen, Carien M., Marchetti, M. Cristina, Manning, M. Lisa and Schwarz, J. M. (2020). Small-scale demixing in confluent biological tissues. Soft Matter, 16 (13). S. 3325 - 3338. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 1744-6848

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Abstract

Surface tension governed by differential adhesion can drive fluid particle mixtures to sort into separate regions, i.e., demix. Does the same phenomenon occur in confluent biological tissues? We begin to answer this question for epithelial monolayers with a combination of theory via a vertex model and experiments on keratinocyte monolayers. Vertex models are distinct from particle models in that the interactions between the cells are shape-based, as opposed to distance-dependent. We investigate whether a disparity in cell shape or size alone is sufficient to drive demixing in bidisperse vertex model fluid mixtures. Surprisingly, we observe that both types of bidisperse systems robustly mix on large lengthscales. On the other hand, shape disparity generates slight demixing over a few cell diameters, a phenomenon we term micro-demixing. This result can be understood by examining the differential energy barriers for neighbor exchanges (T1 transitions). Experiments with mixtures of wild-type and E-cadherin-deficient keratinocytes on a substrate are consistent with the predicted phenomenon of micro-demixing, which biology may exploit to create subtle patterning. The robustness of mixing at large scales, however, suggests that despite some differences in cell shape and size, progenitor cells can readily mix throughout a developing tissue until acquiring means of recognizing cells of different types.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sahu, Preeti UNSPECIFIED orcid.org/0000-0003-1741-1642 UNSPECIFIED Sussman, Daniel M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruebsam, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Mertz, Aaron F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Horsley, Valerie UNSPECIFIED UNSPECIFIED UNSPECIFIED Dufresne, Eric R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Niessen, Carien M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Marchetti, M. Cristina UNSPECIFIED UNSPECIFIED UNSPECIFIED Manning, M. Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, J. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-337444
DOI:	10.1039/c9sm01084j
Journal or Publication Title:	Soft Matter
Volume:	16
Number:	13
Page Range:	S. 3325 - 3338
Date:	2020
Publisher:	ROYAL SOC CHEMISTRY
Place of Publication:	CAMBRIDGE
ISSN:	1744-6848
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTERCELLULAR-ADHESION; DIFFERENTIAL ADHESION; GLASSY DYNAMICS; CELL-SHAPE; SIMULATION; HYPOTHESIS; MECHANICS; MODELS Multiple languages Chemistry, Physical; Materials Science, Multidisciplinary; Physics, Multidisciplinary; Polymer Science Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33744