Universität zu Köln

Kiel, Christina ORCID: 0000-0003-3154-4847, Berber, Patricia, Karlstetter, Marcus, Aslanidis, Alexander, Strunz, Tobias ORCID: 0000-0002-3744-9595, Langmann, Thomas, Grassmann, Felix ORCID: 0000-0003-1390-7528 and Weber, Bernhard H. F. (2020). A Circulating MicroRNA Profile in a Laser-Induced Mouse Model of Choroidal Neovascularization. Int. J. Mol. Sci., 21 (8). BASEL: MDPI. ISSN 1422-0067

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Abstract

Choroidal neovascularization (CNV) is a pathological process in which aberrant blood vessels invade the subretinal space of the mammalian eye. It is a characteristic feature of the prevalent neovascular age-related macular degeneration (nAMD). Circulating microRNAs (cmiRNAs) are regarded as potentially valuable biomarkers for various age-related diseases, including nAMD. Here, we investigated cmiRNA expression in an established laser-induced CNV mouse model. Upon CNV induction in C57Bl/6 mice, blood-derived cmiRNAs were initially determined globally by RNA next generation sequencing, and the most strongly dysregulated cmiRNAs were independently replicated by quantitative reverse transcription PCR (RT-qPCR) in blood, retinal, and retinal pigment epithelium (RPE)/choroidal tissue. Our findings suggest that two miRNAs, mmu-mir-486a-5p and mmur-mir-92a-3p, are consistently dysregulated during CNV formation. Furthermore, in functional in vitro assays, a significant impact of mmu-mir-486a-5p and mmu-mir-92a-3p on murine microglial cell viability was observed, while mmu-mir-92a-3p also showed an impact on microglial mobility. Taken together, we report a robust dysregulation of two miRNAs in blood and RPE/choroid after laser-induced initiation of CNV lesions in mice, highlighting their potential role in pathology and eventual therapy of CNV-associated complications.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kiel, Christina UNSPECIFIED orcid.org/0000-0003-3154-4847 UNSPECIFIED Berber, Patricia UNSPECIFIED UNSPECIFIED UNSPECIFIED Karlstetter, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED Aslanidis, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Strunz, Tobias UNSPECIFIED orcid.org/0000-0002-3744-9595 UNSPECIFIED Langmann, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Grassmann, Felix UNSPECIFIED orcid.org/0000-0003-1390-7528 UNSPECIFIED Weber, Bernhard H. F. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-338176
DOI:	10.3390/ijms21082689
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	21
Number:	8
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL GROWTH-FACTOR; RETINAL-PIGMENT EPITHELIUM; MACULAR DEGENERATION; POTENTIAL BIOMARKERS; NATURAL-HISTORY; IN-VITRO; EXPRESSION; CELLS; ANGIOGENESIS; GENETICS Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33817