Universität zu Köln

Suurvaeli, Jaanus, Whiteley, Andrew R., Zheng, Yichen, Gharbi, Karim ORCID: 0000-0003-1092-4488, Leptin, Maria ORCID: 0000-0001-7097-348X and Wiehe, Thomas ORCID: 0000-0002-8932-2772 (2020). The Laboratory Domestication of Zebrafish: From Diverse Populations to Inbred Substrains. Mol. Biol. Evol., 37 (4). S. 1056 - 1070. OXFORD: OXFORD UNIV PRESS. ISSN 1537-1719

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Abstract

We know from human genetic studies that practically all aspects of biology are strongly influenced by the genetic background, as reflected in the advent of personalizedmedicine. Yet, with few exceptions, this is not taken into account when using laboratory populations as animal model systems for research in these fields. Laboratory strains of zebrafish (Danio rerio) are widely used for research in vertebrate developmental biology, behavior, and physiology, for modeling diseases, and for testing pharmaceutic compounds in vivo. However, all of these strains are derived from artificial bottleneck events and therefore are likely to represent only a fraction of the genetic diversity present within the species. Here, we use restriction site-associated DNA sequencing to genetically characterize wild populations of zebrafish from India, Nepal, and Bangladesh, and to compare themto previously published data on four common laboratory strains. We measured nucleotide diversity, heterozygosity, and allele frequency spectra, and find that wild zebrafish are much more diverse than laboratory strains. Further, in wild zebrafish, there is a clear signal of GC-biased gene conversion that is missing in laboratory strains. We also find that zebrafish populations in Nepal and Bangladesh are most distinct from all other strains studied, making theman attractive subject for future studies of zebrafish population genetics andmolecular ecology. Finally, isolates of the same strains kept in different laboratories show a pattern of ongoing differentiation into genetically distinct substrains. Together, our findings broaden the basis for future genetic, physiological, pharmaceutic, and evolutionary studies in Danio rerio.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Suurvaeli, Jaanus UNSPECIFIED UNSPECIFIED UNSPECIFIED Whiteley, Andrew R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zheng, Yichen UNSPECIFIED UNSPECIFIED UNSPECIFIED Gharbi, Karim UNSPECIFIED orcid.org/0000-0003-1092-4488 UNSPECIFIED Leptin, Maria UNSPECIFIED orcid.org/0000-0001-7097-348X UNSPECIFIED Wiehe, Thomas UNSPECIFIED orcid.org/0000-0002-8932-2772 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-339481
DOI:	10.1093/molbev/msz289
Journal or Publication Title:	Mol. Biol. Evol.
Volume:	37
Number:	4
Page Range:	S. 1056 - 1070
Date:	2020
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1537-1719
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BIASED GENE CONVERSION; RECOMBINATION LANDSCAPE; DANIO-RERIO; WILD; EVOLUTION; VARIANTS; SEQUENCE; MODELS; NUMBER; IDENTIFICATION Multiple languages Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/33948