Universität zu Köln

Kretschmer, Tobias ORCID: 0000-0002-4202-0806, Schulze-Edinghausen, Merle, Turnwald, Eva-Maria, Janoschek, Ruth, Bae-Gartz, Inga ORCID: 0000-0002-4061-503X, Zentis, Peter, Handwerk, Marion, Wohlfarth, Maria, Schauss, Astrid, Hucklenbruch-Rother, Eva, Doetsch, Joerg and Appel, Sarah (2020). Effect of Maternal Obesity in Mice on IL-6 Levels and Placental Endothelial Cell Homeostasis. Nutrients, 12 (2). BASEL: MDPI. ISSN 2072-6643

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Abstract

Obesity during pregnancy is a known health risk for mother and child. Since obesity is associated with increased inflammatory markers, our objectives were to determine interleukin-6 (IL-6) levels in obese mice and to examine the effect of IL-6 on placental endothelial cells. Placentas, blood, and adipose tissue of C57BL/6N mice, kept on high fat diet before and during pregnancy, were harvested at E15.5. Serum IL-6 levels were determined and endothelial cell markers and IL-6 expression were measured by qRT-PCR and western blot. Immunostaining was used to determine surface and length densities of fetal capillary profiles and placental endothelial cell homeostasis. Human placental vein endothelial cells were cultured and subjected to proliferation, apoptosis, senescence, and tube formation assays after stimulation with hyperIL-6. Placental endothelial cell markers were downregulated and the percentage of senescent endothelial cells was higher in the placental exchange zone of obese dams and placental vascularization was strongly reduced. Additionally, maternal IL-6 serum levels and IL-6 protein levels in adipose tissue were increased. Stimulation with hyperIL-6 provoked a dose dependent increase of senescence in cultured endothelial cells without any effects on proliferation or apoptosis. Diet-induced maternal obesity led to an IUGR phenotype accompanied by increased maternal IL-6 serum levels. In the placenta of obese dams, this may result in a disturbed endothelial cell homeostasis and impaired fetal vasculature. Cell culture experiments confirmed that IL-6 is capable of inducing endothelial cell senescence.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kretschmer, Tobias UNSPECIFIED orcid.org/0000-0002-4202-0806 UNSPECIFIED Schulze-Edinghausen, Merle UNSPECIFIED UNSPECIFIED UNSPECIFIED Turnwald, Eva-Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Janoschek, Ruth UNSPECIFIED UNSPECIFIED UNSPECIFIED Bae-Gartz, Inga UNSPECIFIED orcid.org/0000-0002-4061-503X UNSPECIFIED Zentis, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Handwerk, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohlfarth, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Schauss, Astrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Hucklenbruch-Rother, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Doetsch, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Appel, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-346012
DOI:	10.3390/nu12020296
Journal or Publication Title:	Nutrients
Volume:	12
Number:	2
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2072-6643
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HIGH-FAT DIET; INFLAMMATORY CYTOKINES; PREPREGNANCY OBESITY; PREGNANCY; EXPRESSION; GROWTH; ANGIOGENESIS; RECEPTOR; MARKERS; TIE-1 Multiple languages Nutrition & Dietetics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34601