Universität zu Köln

Hilbert, Magdalena, Novero, Mara, Rovenich, Hanna, Mari, Stephane, Grimm, Carolin, Bonfante, Paola and Zuccaro, Alga ORCID: 0000-0002-8026-0114 (2020). MLO Differentially Regulates Barley Root Colonization by Beneficial Endophytic and Mycorrhizal Fungi. Front. Plant Sci., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

Loss-of-function alleles of MLO (Mildew Resistance Locus O) confer broad-spectrum resistance to foliar infections by powdery mildew pathogens. Like pathogens, microbes that establish mutually beneficial relationships with their plant hosts, trigger the induction of some defense responses. Initially, barley colonization by the root endophyte Serendipita indica (syn. Piriformospora indica) is associated with enhanced defense gene expression and the formation of papillae at sites of hyphal penetration attempts. This phenotype is reminiscent of mlo-conditioned immunity in barley leaf tissue and raises the question whether MLO plays a regulatory role in the establishment of beneficial interactions. Here we show that S. indica colonization was significantly reduced in plants carrying mlo mutations compared to wild type controls. The reduction in fungal biomass was associated with the enhanced formation of papillae. Moreover, epidermal cells of S. indica-treated mlo plants displayed an early accumulation of iron in the epidermal layer suggesting increased basal defense activation in the barley mutant background. Correspondingly, the induction of host cell death during later colonization stages was impaired in mlo colonized plants, highlighting the importance of the early biotrophic growth phase for S. indica root colonization. In contrast, the arbuscular mycorrhizal fungus Funneliformis mosseae displayed a similar colonization morphology on mutant and wild type plants. However, the frequency of mycorrhization and number of arbuscules was higher in mlo-5 mutants. These findings suggest that MLO differentially regulates root colonization by endophytic and AM fungi.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hilbert, Magdalena UNSPECIFIED UNSPECIFIED UNSPECIFIED Novero, Mara UNSPECIFIED UNSPECIFIED UNSPECIFIED Rovenich, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Mari, Stephane UNSPECIFIED UNSPECIFIED UNSPECIFIED Grimm, Carolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonfante, Paola UNSPECIFIED UNSPECIFIED UNSPECIFIED Zuccaro, Alga UNSPECIFIED orcid.org/0000-0002-8026-0114 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-348694
DOI:	10.3389/fpls.2019.01678
Journal or Publication Title:	Front. Plant Sci.
Volume:	10
Date:	2020
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POWDERY MILDEW RESISTANCE; GRAMINIS F-SP; PIRIFORMOSPORA-INDICA; DISEASE RESISTANCE; ARABIDOPSIS ROOTS; CELL-WALL; MEDICAGO-TRUNCATULA; MEMBRANE BIOGENESIS; SEBACINA-VERMIFERA; BIOMASS PRODUCTION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/34869