Universität zu Köln

Goettig, Stephan, Walker, Sarah, V, Saleh, Ahmad, Koroska, Florian, Sommer, Julian, Stelzer, Yvonne, Steinmann, Joerg and Hamprecht, Axel ORCID: 0000-0003-1449-5780 (2020). Comparison of nine different selective agars for the detection of carbapenemase-producing Enterobacterales (CPE). Eur. J. Clin. Microbiol. Infect. Dis., 39 (5). S. 923 - 928. NEW YORK: SPRINGER. ISSN 1435-4373

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Abstract

The rapid identification of patients colonized with carbapenem-resistant Enterobacterales (CRE) is important for infection control purposes. Here, we compared and evaluated nine different agars for the detection of carbapenemase-producing Enterobacterales (CPE) from clinical samples. In the study, 69 CPE and 40 carbapenemase-negative isolates were included. Overall, seven commercially available screening agars were assessed: Brilliance CRE (Oxoid), Chromatic CRE (Liofilchem), chromID CARBA and chromID OXA-48 (both bioMerieux), three ESBL agars (Chromatic ESBL [Liofilchem], chromID ESBL [bioMerieux], Brilliance ESBL [Oxoid]), and two agars produced in-house (McCARB and McCARB-T). The sensitivity of CRE agars for CPE detection ranged from 34.8 to 98.6%. Brilliance CRE and McCARB/McCARB-T showed the overall highest sensitivity (98.6 and 97.1%, respectively). OXA-48 producers were the most difficult to detect; only 4/9 agars detected all isolates (McCARB/McCARB-T, Chromatic CRE, ChromID OXA-48). Additionally, all ESBL-negative OXA-48 isolates failed to grow on ESBL screening agars. Specificity ranged from 30 (Brilliance ESBL) to 100% (ChromID OXA-48). The limit of detection for different CPE in spiked stool samples ranged from 1.5 x 10(1) to 1.5 x 10(3) CFU/ml. Overall, Brilliance CRE and the McCARB in-house agars showed the best performance and were able to detect most CPE, including almost all OXA-48. ESBL agars were not suitable for detection of CPE alone, as OXA-48 isolates negative for ESBL were suppressed. The highest sensitivity was achieved by a combination of a CRE agar and an ESBL agar.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goettig, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Walker, Sarah, V UNSPECIFIED UNSPECIFIED UNSPECIFIED Saleh, Ahmad UNSPECIFIED UNSPECIFIED UNSPECIFIED Koroska, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Sommer, Julian UNSPECIFIED UNSPECIFIED UNSPECIFIED Stelzer, Yvonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Steinmann, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-349216
DOI:	10.1007/s10096-019-03786-7
Journal or Publication Title:	Eur. J. Clin. Microbiol. Infect. Dis.
Volume:	39
Number:	5
Page Range:	S. 923 - 928
Date:	2020
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1435-4373
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MEDIA; TESTS Multiple languages Infectious Diseases; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34921