Universität zu Köln

Nattramilarasu, Praveen Kumar ORCID: 0000-0002-8212-7368, Buecker, Roland, de Sa, Fabia Daniela Lobo, Fromm, Anja, Nagel, Oliver, Lee, In-Fah Maria, Butkevych, Eduard, Mousavi, Soraya, Genger, Claudia, Klove, Sigri, Heimesaat, Markus M., Bereswill, Stefan, Schweiger, Michal R., Nielsen, Hans Linde, Troeger, Hanno and Schulzke, Joerg-Dieter (2020). Campylobacter concisus Impairs Sodium Absorption in Colonic Epithelium via ENaC Dysfunction and Claudin-8 Disruption. Int. J. Mol. Sci., 21 (2). BASEL: MDPI. ISSN 1422-0067

Full text not available from this repository.

Abstract

The epithelial sodium channel (ENaC) can increase the colonic absorptive capacity for salt and water. Campylobacter concisus is a common pathogenic epsilonproteobacterium, causing enteritis and diarrhea. It can induce barrier dysfunction in the intestine, but its influence on intestinal transport function is still unknown. Therefore, our study aimed to characterize C. concisus effects on ENaC using the HT-29/B6-GR/MR (epithelial cell line HT-29/B6 transfected with glucocorticoid and mineralocorticoid receptors) cell model and mouse colon. In Ussing chambers, C. concisus infection inhibited ENaC-dependent Na+ transport as indicated by a reduction in amiloride-sensitive short circuit current (-55%, n = 15, p < 0.001). This occurred via down-regulation of beta- and gamma-ENaC mRNA expression and ENaC ubiquitination due to extracellular signal-regulated kinase (ERK)1/2 activation, predicted by Ingenuity Pathway Analysis (IPA). In parallel, C. concisus reduced the expression of the sealing tight junction (TJ) protein claudin-8 and induced claudin-8 redistribution off the TJ domain of the enterocytes, which facilitates the back leakage of Na+ ions into the intestinal lumen. In conclusion, C. concisus caused ENaC dysfunction via interleukin-32-regulated ERK1/2, as well as claudin-8-dependent barrier dysfunction-both of which contribute to Na+ malabsorption and diarrhea.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nattramilarasu, Praveen Kumar UNSPECIFIED orcid.org/0000-0002-8212-7368 UNSPECIFIED Buecker, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED de Sa, Fabia Daniela Lobo UNSPECIFIED UNSPECIFIED UNSPECIFIED Fromm, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Nagel, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Lee, In-Fah Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Butkevych, Eduard UNSPECIFIED UNSPECIFIED UNSPECIFIED Mousavi, Soraya UNSPECIFIED UNSPECIFIED UNSPECIFIED Genger, Claudia UNSPECIFIED UNSPECIFIED UNSPECIFIED Klove, Sigri UNSPECIFIED UNSPECIFIED UNSPECIFIED Heimesaat, Markus M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bereswill, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schweiger, Michal R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Nielsen, Hans Linde UNSPECIFIED UNSPECIFIED UNSPECIFIED Troeger, Hanno UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulzke, Joerg-Dieter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-349279
DOI:	10.3390/ijms21020373
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	21
Number:	2
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICE LACKING; BACTEROIDES-GRACILIS; EIKENELLA-CORRODENS; TETRAZOLIUM SALT; BARRIER FUNCTION; WOLINELLA-RECTA; NA+ ABSORPTION; EXPRESSION; CHANNEL; INTESTINE Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34927