Universität zu Köln

Burberg, Christian ORCID: 0000-0002-2571-6908, Petzoldt, Thomas ORCID: 0000-0002-4951-6468 and von Elert, Eric (2020). Phosphate Limitation Increases Content of Protease Inhibitors in the Cyanobacterium Microcystis aeruginosa. Toxins, 12 (1). BASEL: MDPI. ISSN 2072-6651

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Abstract

Increased anthropogenic nutrient input has led to eutrophication of lakes and ponds, resulting worldwide in more frequent and severe cyanobacterial blooms. In particular, enhanced availability of phosphorus (P) can promote cyanobacterial mass developments and may affect the content of secondary metabolites in cyanobacteria, such as protease inhibitors (PIs). PIs are common among cyanobacteria and have been shown to negatively affect herbivorous zooplankton. Here, we test the hypothesis that P-limitation reduces the growth of Microcystis, but increases the content of PIs. In batch culture experiments with eight different initial phosphate concentrations (5-75 mu M) we determined growth, stoichiometry, and PI content of Microcystis aeruginosa NIVA Cya 43. This strain produces the protease inhibitor BN920 that is converted by chlorination to CP954, which constitutes the major PI in this strain. C:N:P-ratios of the biomass indicated variation of P-limitation with treatment and time. When normalized to biomass, the PI content varied up to nearly nineteen-fold with treatment and time and was highest in the low-P treatments, especially during the mid-exponential growth phase. However, these effects were alleviated under nitrogen co-limitation. The content of CP954 showed an inverse u-shaped response to growth rate and C:N-ratio of the cyanobacterial biomass, whereas it increased with cyanobacterial C:P. The results indicate that P-limitation supports a higher content of defensive PIs and may indirectly foster cyanobacterial blooms by increasing the negative interference of cyanobacteria with their consumers.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Burberg, Christian UNSPECIFIED orcid.org/0000-0002-2571-6908 UNSPECIFIED Petzoldt, Thomas UNSPECIFIED orcid.org/0000-0002-4951-6468 UNSPECIFIED von Elert, Eric UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-351408
DOI:	10.3390/toxins12010033
Journal or Publication Title:	Toxins
Volume:	12
Number:	1
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2072-6651
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARBON-NUTRIENT BALANCE; DAPHNIA-MAGNA; FRESH-WATER; NITROGEN; GROWTH; LAKES; EUTROPHICATION; EXPRESSION; TOLERANCE; TOXICITY Multiple languages Food Science & Technology; Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/35140