Universität zu Köln

Huetten, Marion, Geukes, Melanie, Misas-Villamil, Johana C., van der Hoorn, Renier A. L., Grundler, Florian M. W. and Siddique, Shahid ORCID: 0000-0001-7503-4318 (2015). Activity profiling reveals changes in the diversity and activity of proteins in Arabidopsis roots in response to nematode infection. Plant Physiol. Biochem., 97. S. 36 - 44. PARIS: ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER. ISSN 0981-9428

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Abstract

Cyst nematodes are obligate, sedentary endoparasites with a highly specialised biology and a huge economic impact in agriculture. Successful parasitism involves morphological and physiological modifications of the host cells which lead to the formation of specialised syncytial feeding structures in roots. The development of the syncytium is aided by a cocktail of nematode effectors that manipulate the host plant activities in a complex network of interactions through post-translational modifications. Traditional transcriptomic and proteomic approaches cannot display this functional proteomic information. Activity-based protein profiling (ABPP) is a powerful technology that can be used to investigate the activity of the proteome through activity-based probes. To better understand the functional proteomics of syncytium, ABPP was conducted on syncytia induced by the beet cyst nematode Heterodera schachtii in Arabidopsis roots. Our results demonstrated that the activity of several enzymes is differentially regulated in the syncytium compared to the control roots. Among those specifically activated in the syncytium are a putative S-formyl-glutathione hydrolase (SFGH), a putative methylesterase (MES) and two unidentified enzymes. In contrast, the activities of vacuolar processing enzymes (VPEs) are specifically suppressed in the syncytium. Competition labelling, quantitative gene expression and T-DNA knock-out mutants were used to further characterise the roles of the differentially regulated enzymes during plant nematode interaction. In conclusion, our study will open the door to generate a comprehensive and integrated view of the host-pathogen warfare that results in the formation of long-term feeding sites for pathogens. (C) 2015 Elsevier Masson SAS. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Huetten, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Geukes, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Misas-Villamil, Johana C. UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Hoorn, Renier A. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grundler, Florian M. W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Siddique, Shahid UNSPECIFIED orcid.org/0000-0001-7503-4318 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-385114
DOI:	10.1016/j.plaphy.2015.09.008
Journal or Publication Title:	Plant Physiol. Biochem.
Volume:	97
Page Range:	S. 36 - 44
Date:	2015
Publisher:	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Place of Publication:	PARIS
ISSN:	0981-9428
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VACUOLAR PROCESSING ENZYME; ACTIVITY-BASED PROBES; HETERODERA-SCHACHTII; CYSTEINE PROTEASES; CELL-DEATH; DISEASE RESISTANCE; SERINE HYDROLASES; MODEL HOST; THALIANA; VPE Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38511