Boch, T., Reinwald, M., Postina, P., Cornely, O. A., Vehreschild, J. J., Heussel, C. P., Heinz, W. J., Hoenigl, M., Eigl, S., Lehrnbecher, T., Hahn, J., Claus, B., Lauten, M., Egerer, G., Mueller, M. C., Will, S., Merker, N., Hofmann, W. -K., Buchheidt, D. and Spiess, B. (2015). Identification of invasive fungal diseases in immunocompromised patients by combining an Aspergillus specific PCR with a multifungal DNA-microarray from primary clinical samples. Mycoses, 58 (12). S. 735 - 746. HOBOKEN: WILEY. ISSN 1439-0507

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Abstract

The increasing incidence of invasive fungal diseases (IFD), most of all invasive aspergillosis (IA) in immunocompromised patients emphasises the need to improve the diagnostic tools for detection of fungal pathogens. We investigated the diagnostic performance of a multifungal DNA-microarray detecting 15 different fungi [Aspergillus, Candida, Fusarium, Mucor, Rhizopus, Scedosporium and Trichosporon species (spp.)] in addition to an Aspergillus specific polymerase chain reaction (PCR) assay. Biopsies, bronchoalveolar lavage and peripheral blood samples of 133 immunocompromised patients (pts) were investigated by a multifungal DNA-microarray as well as a nested Aspergillus specific PCR assay. Patients had proven (n=18), probable (n=29), possible (n=48) and no IFD (n=38) and were mostly under antifungal therapy at the time of sampling. The results were compared to culture, histopathology, imaging and serology, respectively. For the non-Aspergillus IFD the microarray analysis yielded in all samples a sensitivity of 64% and a specificity of 80%. Best results for the detection of all IFD were achieved by combining DNA-microarray and Aspergillus specific PCR in biopsy samples (sensitivity 79%; specificity 71%). The molecular assays in combination identify genomic DNA of fungal pathogens and may improve identification of causative pathogens of IFD and help overcoming the diagnostic uncertainty of culture and/or histopathology findings, even during antifungal therapy.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Boch, T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Reinwald, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Postina, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cornely, O. A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vehreschild, J. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heussel, C. P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heinz, W. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoenigl, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eigl, S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lehrnbecher, T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hahn, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Claus, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lauten, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Egerer, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mueller, M. C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Will, S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Merker, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, W. -K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Buchheidt, D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Spiess, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-386262
DOI: 10.1111/myc.12424
Journal or Publication Title: Mycoses
Volume: 58
Number: 12
Page Range: S. 735 - 746
Date: 2015
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1439-0507
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
POLYMERASE-CHAIN-REACTION; BRONCHOALVEOLAR LAVAGE SAMPLES; REAL-TIME PCR; HEMATOLOGICAL MALIGNANCIES; PULMONARY ASPERGILLOSIS; TRANSPLANT RECIPIENTS; MOLD INFECTIONS; CANCER-PATIENTS; GERMAN SOCIETY; REACTION ASSAYMultiple languages
Dermatology; MycologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/38626

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