Universität zu Köln

Roth, Heike, Samereier, Matthias, Trommler, Gudrun, Noegel, Angelika A., Schleicher, Michael and Mueller-Taubenberger, Annette (2015). Balanced cortical stiffness is important for efficient migration of Dictyostelium cells in confined environments. Biochem. Biophys. Res. Commun., 467 (4). S. 730 - 736. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2104

Full text not available from this repository.

Abstract

Dictyostelium discoideum cells resemble in many aspects human leukocytes and serve as a model to study actin cytoskeleton dynamics and cell migration of highly motile cells. Dictyostelium cells deficient in the actin-binding protein filamin (ddFLN) showed a surprisingly subtle change in phenotype with no or only minor effects in single cell motility. These findings were in contrast to the strong actin-crosslinking activities measured for filamin in vitro. In the present study, we set out to revisit the role of ddFLN in cell migration. For this purpose, we examined migration of wild-type, ddFLN-null and ddFLN-overexpressing cells under different conditions. In addition to cyclic-AMP chemotaxis assays using micropipettes, we explored cell migration under more confined conditions: an under-agarose 2D assay and a 3D assay employing a collagen matrix that was adapted from assays for leukocytes. Using 3D migration conditions, cells deficient in ddFLN displayed only a minor impairment of motility, similar to the results obtained for migration in 2D. However, cells overexpressing ddFLN showed a remarkable decrease in the speed of migration in particular in 3D environments. We suggest that these results are in line with an increased stiffening of the cortex due to the crosslinking activity of overexpressed ddFLN. Our conclusion is that the absolute level of ddFLN is critical for efficient migration. Furthermore, our results show that under conditions of increased mechanical stress, Dictyostelium cells, like leukocytes, switch to a bleb-based mode of movement. (C) 2015 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Roth, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED Samereier, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Trommler, Gudrun UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schleicher, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller-Taubenberger, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-386717
DOI:	10.1016/j.bbrc.2015.10.073
Journal or Publication Title:	Biochem. Biophys. Res. Commun.
Volume:	467
Number:	4
Page Range:	S. 730 - 736
Date:	2015
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2104
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GELATION FACTOR ABP-120; CROSS-LINKING PROTEINS; ACTIN-BINDING PROTEIN; ALPHA-ACTININ; WILD-TYPE; MOTILITY; DEFECTS; CYTOSKELETAL; FILAMINS; LACKING Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38671