Universität zu Köln

vom Dorp, Katharina, Hoelzl, Georg, Plohmann, Christian, Eisenhut, Marion, Abraham, Marion, Weber, Andreas P. M., Hanson, Andrew D. and Doermann, Peter (2015). Remobilization of Phytol from Chlorophyll Degradation Is Essential for Tocopherol Synthesis and Growth of Arabidopsis. Plant Cell, 27 (10). S. 2846 - 2860. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1531-298X

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Abstract

Phytol from chlorophyll degradation can be phosphorylated to phytyl-phosphate and phytyl-diphosphate, the substrate for tocopherol (vitamin E) synthesis. A candidate for the phytyl-phosphate kinase from Arabidopsis thaliana (At1g78620) was identified via a phylogeny-based approach. This gene was designated VITAMIN E DEFICIENT6 (VTE6) because the leaves of the Arabidopsis vte6 mutants are tocopherol deficient. The vte6 mutant plants are incapable of photoautotrophic growth. Phytol and phytyl-phosphate accumulate, and the phytyl-diphosphate content is strongly decreased in vte6 leaves. Phytol feeding and enzyme assays with Arabidopsis and recombinant Escherichia coli cells demonstrated that VTE6 has phytyl-P kinase activity. Overexpression of VTE6 resulted in increased phytyl-diphosphate and tocopherol contents in seeds, indicating that VTE6 encodes phytyl-phosphate kinase. The severe growth retardation of vte6 mutants was partially rescued by introducing the phytol kinase mutation vte5. Double mutant plants (vte5 vte6) are tocopherol deficient and contain more chlorophyll, but reduced amounts of phytol and phytyl-phosphate compared with vte6 mutants, suggesting that phytol or phytyl-phosphate are detrimental to plant growth. Therefore, VTE6 represents the missing phytyl-phosphate kinase, linking phytol release from chlorophyll with tocopherol synthesis. Moreover, tocopherol synthesis in leaves depends on phytol derived from chlorophyll, not on de novo synthesis of phytyl-diphosphate from geranylgeranyl-diphosphate.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code vom Dorp, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoelzl, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Plohmann, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Eisenhut, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Abraham, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanson, Andrew D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Doermann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-392292
DOI:	10.1105/tpc.15.00395
Journal or Publication Title:	Plant Cell
Volume:	27
Number:	10
Page Range:	S. 2846 - 2860
Date:	2015
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1531-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SYNECHOCYSTIS SP PCC-6803; VITAMIN-E BIOSYNTHESIS; HOMOGENTISATE PHYTYLTRANSFERASE; CHLOROPLAST ENVELOPE; METABOLISM; BREAKDOWN; IDENTIFICATION; PHEOPHORBIDE; DESICCATION; ANNOTATION Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39229