Universität zu Köln

Endepols, Heike ORCID: 0000-0002-6166-4818, Mertgens, Hanna, Backes, Heiko, Himmelreich, Uwe ORCID: 0000-0002-2060-8895, Neumaier, Bernd ORCID: 0000-0001-5425-3116, Graf, Rudolf and Mies, Guenter (2015). Longitudinal assessment of infarct progression, brain metabolism and behavior following anterior cerebral artery occlusion in rats. J. Neurosci. Methods, 253. S. 279 - 292. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1872-678X

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Abstract

Background: Stroke patients suffering from occlusion of the anterior cerebral artery (ACAo) develop cognitive and executive deficits. Experimental models to investigate such functional impairments and recovery are rare and not satisfyingly validated. New method: We stereotactically injected the vasoconstrictor endothelin-1 (ET-1) close to the ACA of rats and assessed magnitude and course of CBF reduction using [C-14]iodoantipyrine autoradiography and [O-15]H2O-PET. [F-18]FDG-PET and 12-weighted MRI determined regional metabolic and structural alterations. To test cognitive and executive functions, we analyzed decision-making in a food-carrying task, spatial working memory in a spontaneous alternation task and anxiety in an elevated plus maze test before and 1 month after ACAo. Results: CBF decreased immediately after ET-1 injection, started to recover 1-2 h and returned to control 4h thereafter. Metabolic and structural lesions developed permanently in the ACA territory. Hypometabolism occurring bilaterally in the piriform region may reflect diaschisis. Behavioral testing after ACAo revealed context-dependent changes in decision making, exploratory activity and walking speed, as well as decreased anxiety and spatial working memory. Comparison with existing method(s): Aside from modeling a known entity of stroke patients, ACAo in rats allows to longitudinally study deterioration of cognitive and executive function without major interference by disturbed primary motor function. It complements therefore stroke research since common models using middle cerebral artery occlusion (MCAo) all affect motor function severely. Conclusion: The established ACAo model in rats effectively reflects deficits characteristic for ACA stroke in humans. It is furthermore highly suitable for longitudinal assessment of cognitive and executive functions. (C) 2014 The Authors. Published by Elsevier B.V.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Endepols, Heike UNSPECIFIED orcid.org/0000-0002-6166-4818 UNSPECIFIED Mertgens, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Backes, Heiko UNSPECIFIED UNSPECIFIED UNSPECIFIED Himmelreich, Uwe UNSPECIFIED orcid.org/0000-0002-2060-8895 UNSPECIFIED Neumaier, Bernd UNSPECIFIED orcid.org/0000-0001-5425-3116 UNSPECIFIED Graf, Rudolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Mies, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-392574
DOI:	10.1016/j.jneumeth.2014.11.003
Journal or Publication Title:	J. Neurosci. Methods
Volume:	253
Page Range:	S. 279 - 292
Date:	2015
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1872-678X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MEDIAL PREFRONTAL CORTEX; FOOD-CARRYING TASK; RATTUS-NORVEGICUS; ISCHEMIC-STROKE; SPONTANEOUS-ALTERNATION; TERRITORY INFARCTION; FORAGING RATS; F-18-FDG PET; BLOOD-FLOW; MODEL Multiple languages Biochemical Research Methods; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39257