Universität zu Köln

Vassella, Erik, Langsch, Stephanie, Dettmer, Matthias S., Schlup, Cornelia, Neuenschwander, Maja, Frattini, Milo, Gugger, Mathias and Schaefer, Stephan C. (2015). Molecular profiling of lung adenosquamous carcinoma: hybrid or genuine type? Oncotarget, 6 (27). S. 23905 - 23917. ORCHARD PARK: IMPACT JOURNALS LLC. ISSN 1949-2553

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Abstract

Lung adenosquamous carcinoma is a particular subtype of non-small cell lung carcinoma that is defined by the coexistence of adenocarcinoma and squamous cell carcinoma components. The aim of this study was to assess the mutational profile in each component of 16 adenosquamous carcinoma samples from a Caucasian population by a combination of next generation sequencing using the cancer hotspot panel as well as the colon and lung cancer panel and FISH. Identified mutations were confirmed by Sanger sequencing of DNA from cancer cells of each component collected by Laser Capture microdissection. Mutations typical for adenocarcinoma as well as squamous cell carcinoma were identified. Driver mutations were predominantly in the trunk suggesting a monoclonal origin of adenosquamous carcinoma. Most remarkably, EGFR mutations and mutations in the PI3K signaling pathway, which accounted for 30% and 25% of tumors respectively, were more prevalent while KRAS mutations were less prevalent than expected for a Caucasian population. Surprisingly, expression of classifier miR-205 was intermediate between that of classical adenocarcinoma and squamous cell carcinoma suggesting that adenosquamous carcinoma is a transitional stage between these tumor types. The high prevalence of therapy-relevant targets opens new options of therapeutic intervention for adenosquamous carcinoma patients.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Vassella, Erik UNSPECIFIED UNSPECIFIED UNSPECIFIED Langsch, Stephanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Dettmer, Matthias S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlup, Cornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Neuenschwander, Maja UNSPECIFIED UNSPECIFIED UNSPECIFIED Frattini, Milo UNSPECIFIED UNSPECIFIED UNSPECIFIED Gugger, Mathias UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaefer, Stephan C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-392924
DOI:	10.18632/oncotarget.4163
Journal or Publication Title:	Oncotarget
Volume:	6
Number:	27
Page Range:	S. 23905 - 23917
Date:	2015
Publisher:	IMPACT JOURNALS LLC
Place of Publication:	ORCHARD PARK
ISSN:	1949-2553
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SQUAMOUS-CELL CARCINOMA; GROWTH-FACTOR RECEPTOR; CLINICOPATHOLOGICAL CHARACTERISTICS; CANCER; ADENOCARCINOMA; MUTATIONS; KRAS; DIFFERENTIATION; HETEROGENEITY; INHIBITORS Multiple languages Oncology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39292