Ramalingam, Nagendran, Franke, Christof, Jaschinski, Evelin, Winterhoff, Moritz, Lu, Yao, Bruehmann, Stefan, Junemann, Alexander, Meier, Helena, Noegel, Angelika A., Weber, Igor ORCID: 0000-0003-4296-3166, Zhao, Hongxia ORCID: 0000-0002-1606-5621, Merkel, Rudolf ORCID: 0000-0003-3178-3282, Schleicher, Michael and Faix, Jan (2015). A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement. Nat. Commun., 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Cell migration is driven by the establishment of disparity between the cortical properties of the softer front and the more rigid rear allowing front extension and actomyosin-based rear contraction. However, how the cortical actin meshwork in the rear is generated remains elusive. Here we identify the mDia1-like formin A (ForA) from Dictyostelium discoideum that generates a subset of filaments as the basis of a resilient cortical actin sheath in the rear. Mechanical resistance of this actin compartment is accomplished by actin crosslinkers and IQGAP-related proteins, and is mandatory to withstand the increased contractile forces in response to mechanical stress by impeding unproductive blebbing in the rear, allowing efficient cell migration in two-dimensional-confined environments. Consistently, ForA supresses the formation of lateral protrusions, rapidly relocalizes to new prospective ends in repolarizing cells and is required for cortical integrity. Finally, we show that ForA utilizes the phosphoinositide gradients in polarized cells for subcellular targeting.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ramalingam, NagendranUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franke, ChristofUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jaschinski, EvelinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Winterhoff, MoritzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lu, YaoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bruehmann, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Junemann, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meier, HelenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Noegel, Angelika A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weber, IgorUNSPECIFIEDorcid.org/0000-0003-4296-3166UNSPECIFIED
Zhao, HongxiaUNSPECIFIEDorcid.org/0000-0002-1606-5621UNSPECIFIED
Merkel, RudolfUNSPECIFIEDorcid.org/0000-0003-3178-3282UNSPECIFIED
Schleicher, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Faix, JanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-395778
DOI: 10.1038/ncomms9496
Journal or Publication Title: Nat. Commun.
Volume: 6
Date: 2015
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CELL MOTILITY; RAC1 GTPASES; MYOSIN-II; PROTEIN; CYTOSKELETON; MIGRATION; IQGAP1; CORTEXILLINS; POLARIZATION; CYTOKINESISMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/39577

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