Universität zu Köln

Wunsch, Marie, Zhang, Wenji, Hanson, Jodi, Caspell, Richard, Karulin, Alexey Y., Recks, Mascha S., Kuerten, Stefanie, Sundararaman, Srividya and Lehmann, Paul V. (2015). Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity. Viruses-Basel, 7 (8). S. 4414 - 4438. BASEL: MDPI AG. ISSN 1999-4915

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Abstract

Most humans become infected with human cytomegalovirus (HCMV). Typically, the immune system controls the infection, but the virus persists and can reactivate in states of immunodeficiency. While substantial information is available on the contribution of CD8 T cells and antibodies to anti-HCMV immunity, studies of the T(H)1, T(H)2, and T(H)17 subsets have been limited by the low frequency of HCMV-specific CD4 T cells in peripheral blood mononuclear cell (PBMC). Using the enzyme-linked Immunospot((R)) assay (ELISPOT) that excels in low frequency measurements, we have established these in a sizable cohort of healthy HCMV controllers. Cytokine recall responses were seen in all seropositive donors. Specifically, interferon (IFN)- and/or interleukin (IL)-17 were seen in isolation or with IL-4 in all test subjects. IL-4 recall did not occur in isolation. While the ratios of T(H)1, T(H)2, and T(H)17 cells exhibited substantial variations between different individuals these ratios and the frequencies were relatively stable when tested in samples drawn up to five years apart. IFN- and IL-2 co-expressing polyfunctional cells were seen in most subjects. Around half of the HCMV-specific CD4 cells were in a reversible state of exhaustion. The data provided here established the T(H)1, T(H)2, and T(H)17 characteristic of the CD4 cells that convey immune protection for successful immune surveillance against which reactivity can be compared when the immune surveillance of HCMV fails.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wunsch, Marie UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Wenji UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanson, Jodi UNSPECIFIED UNSPECIFIED UNSPECIFIED Caspell, Richard UNSPECIFIED UNSPECIFIED UNSPECIFIED Karulin, Alexey Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Recks, Mascha S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuerten, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Sundararaman, Srividya UNSPECIFIED UNSPECIFIED UNSPECIFIED Lehmann, Paul V. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397074
DOI:	10.3390/v7082828
Journal or Publication Title:	Viruses-Basel
Volume:	7
Number:	8
Page Range:	S. 4414 - 4438
Date:	2015
Publisher:	MDPI AG
Place of Publication:	BASEL
ISSN:	1999-4915
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHRONIC VIRAL-INFECTION; MEMORY CELLS; CMV INFECTION; HCV INFECTION; B-CELLS; CYTOMEGALOVIRUS; ELISPOT; EXHAUSTION; ACTIVATION; SIGNATURE Multiple languages Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39707