Universität zu Köln

Hooper, Scott L., Thuma, Jeffrey B., Guschlbauer, Christoph, Schmidt, Joachim and Bueschges, Ansgar (2015). Cell dialysis by sharp electrodes can cause nonphysiological changes in neuron properties. J. Neurophysiol., 114 (2). S. 1255 - 1272. BETHESDA: AMER PHYSIOLOGICAL SOC. ISSN 1522-1598

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Abstract

We recorded from lobster and leech neurons with two sharp electrodes filled with solutions often used with these preparations (lobster: 0.6 M K2SO4 or 2.5 M KAc; leech: 4 M KAc), with solutions approximately matching neuron cytoplasm ion concentrations, and with 6.5 M KAc (lobster, leech) and 0.6 M KAc (lobster). We measured membrane potential, input resistance, and transient and sustained depolarization-activated outward current amplitudes in leech and these neuron properties and hyperpolarization-activated current time constant in lobster, every 10 min for 60 min after electrode penetration. Neuron properties varied with electrode fill. For fills with molarities >= 2.5 M, neuron properties also varied strongly with time after electrode penetration. Depending on the property being examined, these variations could be large. In leech, cell size also increased with noncytoplasmic fills. The changes in neuron properties could be due to the ions being injected from the electrodes during current injection. We tested this possibility in lobster with the 2.5 M KAc electrode fill by making measurements only 10 and 60 min after penetration. Neuron properties still changed, although the changes were less extreme. Making measurements every 2 min showed that the time-dependent variations in neuron properties occurred in concert with each other. Neuron property changes with high molarity electrode-fill solutions were great enough to decrease neuron firing strongly. An experiment with C-14-glucose electrode fill confirmed earlier work showing substantial leak from sharp electrodes. Sharp electrode work should thus be performed with cytoplasm- matched electrode fills.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hooper, Scott L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Thuma, Jeffrey B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Guschlbauer, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED Bueschges, Ansgar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397280
DOI:	10.1152/jn.01010.2014
Journal or Publication Title:	J. Neurophysiol.
Volume:	114
Number:	2
Page Range:	S. 1255 - 1272
Date:	2015
Publisher:	AMER PHYSIOLOGICAL SOC
Place of Publication:	BETHESDA
ISSN:	1522-1598
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CENTRAL-NERVOUS-SYSTEM; ACTIVITY-DEPENDENT REGULATION; LEECH HIRUDO-MEDICINALIS; STOMATOGASTRIC GANGLION; RETZIUS CELLS; CULTURED NEURONS; MODEL NEURONS; GLIAL-CELLS; MICROELECTRODES; CONSTRUCTION Multiple languages Neurosciences; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39728