Universität zu Köln

Montesinos-Rongen, Manuel, Purschke, Frauke G., Brunn, Anna, May, Caroline, Nordhoff, Eckhard, Marcus, Katrin and Deckert, Martina (2015). Primary Central Nervous System (CNS) Lymphoma B Cell Receptors Recognize CNS Proteins. J. Immunol., 195 (3). S. 1312 - 1320. BETHESDA: AMER ASSOC IMMUNOLOGISTS. ISSN 1550-6606

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Abstract

Primary lymphoma of the CNS (PCNSL) is a diffuse large B cell lymphoma confined to the CNS. To elucidate its peculiar organ tropism, we generated recombinant Abs (recAbs) identical to the BCR of 23 PCNSLs from immunocompetent patients. Although none of the recAbs showed self-reactivity upon testing with common autoantigens, they recognized 1547 proteins present on a large-scale protein microarray, indicating polyreactivity. Interestingly, proteins (GRINL1A, centaurin-alpha, BAIAP2) recognized by the recAbs are physiologically expressed by CNS neurons. Furthermore, 87% (20/23) of the recAbs, including all Abs derived from IGHV4-34 using PCNSL, recognized galectin-3, which was upregulated on microglia/macrophages, astrocytes, and cerebral endothelial cells upon CNS invasion by PCNSL. Thus, PCNSL Ig may recognize CNS proteins as self-Ags. Their interaction may contribute to BCR signaling with sustained NF-kappa B activation and, ultimately, may foster tumor cell proliferation and survival. These data may also explain, at least in part, the affinity of PCNSL cells for the CNS.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Montesinos-Rongen, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Purschke, Frauke G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brunn, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED May, Caroline UNSPECIFIED UNSPECIFIED UNSPECIFIED Nordhoff, Eckhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Marcus, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Deckert, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397736
DOI:	10.4049/jimmunol.1402341
Journal or Publication Title:	J. Immunol.
Volume:	195
Number:	3
Page Range:	S. 1312 - 1320
Date:	2015
Publisher:	AMER ASSOC IMMUNOLOGISTS
Place of Publication:	BETHESDA
ISSN:	1550-6606
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HIV-NEGATIVE PATIENTS; GALECTIN-3 EXPRESSION; BIOMARKER DISCOVERY; AUTOREACTIVITY; REPERTOIRE; PRECURSORS; INFECTION; SEQUENCES; ABSENCE; DISEASE Multiple languages Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39773