Universität zu Köln

Wiebe, Helene, Guerlebeck, Doreen, Gross, Jana, Dreck, Katrin, Pannen, Derk, Ewers, Christa, Wieler, Lothar H. ORCID: 0000-0003-4365-3717 and Schnetz, Karin ORCID: 0000-0003-4693-6202 (2015). YjjQ Represses Transcription of flhDC and Additional Loci in Escherichia coli. J. Bacteriol., 197 (16). S. 2713 - 2721. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-5530

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Abstract

The presumptive transcriptional regulator YjjQ has been identified as being virulence associated in avian pathogenic Escherichia coli (APEC). In this work, we characterize YjjQ as transcriptional repressor of the flhDC operon, encoding the master regulator of flagellar synthesis, and of additional loci. The latter include gfc (capsule 4 synthesis), ompC (outer membrane porin C), yfiRNB (regulated c-di-GMP synthesis), and loci of poorly defined function (ybhL and ymiA-yciX). We identify the YjjQ DNA-binding sites at the flhDC and gfc promoters and characterize a DNA-binding sequence motif present at all promoters found to be repressed by YjjQ. At the flhDC promoter, the YjjQ DNA-binding site overlaps the RcsA-RcsB DNA-binding site. RcsA-RcsB likewise represses the flhDC promoter, but the repression by YjjQ and that by RcsA-RcsB are independent of each other. These data suggest that YjjQ is an additional regulator involved in the complex control of flhDC at the level of transcription initiation. Furthermore, we show that YjjQ represses motility of the E. coli K-12 laboratory strain and of uropathogenic E. coli (UPEC) strains CFT073 and 536. Regulation of flhDC, yfiRNB, and additional loci by YjjQ may be features relevant for pathogenicity. IMPORTANCE Escherichia coli is a commensal and pathogenic bacterium causing intra-and extraintestinal infections in humans and farm animals. The pathogenicity of E. coli strains is determined by their particular genome content, which includes essential and associated virulence factors that control the cellular physiology in the host environment. However, the gene pools of commensal and pathogenic E. coli are not clearly differentiated, and the function of virulence-associated loci needs to be characterized. In this study, we characterize the function of yjjQ, encoding a transcription regulator that was identified as being virulence associated in avian pathogenic E. coli (APEC). We characterize YjjQ as transcriptional repressor of flagellar motility and of additional loci related to pathogenicity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wiebe, Helene UNSPECIFIED UNSPECIFIED UNSPECIFIED Guerlebeck, Doreen UNSPECIFIED UNSPECIFIED UNSPECIFIED Gross, Jana UNSPECIFIED UNSPECIFIED UNSPECIFIED Dreck, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Pannen, Derk UNSPECIFIED UNSPECIFIED UNSPECIFIED Ewers, Christa UNSPECIFIED UNSPECIFIED UNSPECIFIED Wieler, Lothar H. UNSPECIFIED orcid.org/0000-0003-4365-3717 UNSPECIFIED Schnetz, Karin UNSPECIFIED orcid.org/0000-0003-4693-6202 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-397828
DOI:	10.1128/JB.00263-15
Journal or Publication Title:	J. Bacteriol.
Volume:	197
Number:	16
Page Range:	S. 2713 - 2721
Date:	2015
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-5530
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NS-MEDIATED REPRESSION; H-NS; RCS PHOSPHORELAY; ANTIBIOTIC-RESISTANCE; FLAGELLAR EXPRESSION; ENTERIC BACTERIA; ENVELOPE STRESS; GENE-EXPRESSION; BGLJ-RCSB; E. COLI Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/39782