Universität zu Köln

Zhu, Zhuoran, Tan, Zhoubin, Li, Yan, Luo, Hongyan, Hu, Xinwu, Tang, Ming, Hescheler, Juergen, Mu, Yangling and Zhang, Lanqiu (2015). Docosahexaenoic acid alters Gs alpha localization in lipid raft and potentiates adenylate cyclase. Nutrition, 31 (7-8). S. 1025 - 1031. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1873-1244

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Abstract

Objective: Supplementation with docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid (PUFA), recently has become popular for the amelioration of depression; however the molecular mechanism of DHA action remains unclear. The aim of this study was to investigate the mechanism underlying the antidepressant effect of DHA by evaluating Gs alpha localization in lipid raft and the activity of adenylate cyclase in an in vitro glioma cell model. Methods: Lipid raft fractions from C6 glioma cells treated chronically with DHA were isolated by sucrose gradient ultracentrifugation. The content of Gs alpha in lipid raft was analyzed by immunoblotting and colocalization of Gs alpha with lipid raft was subjected to confocal microscopic analysis. The intracellular cyclic adenosine monophosphate (CAMP) level was determined by cAMP immunoassay kit. Results: DHA decreased the amount of Gs alpha in lipid raft, whereas whole cell lysate Gs alpha was not changed. Confocal microscopic analysis demonstrated that colocalization of Gs alpha with lipid raft was decreased, whereas DHA increased intracellular CAMP accumulation in a dose-dependent manner. Interestingly, we found that DHA increased the lipid raft level, instead of disrupting it. Conclusions: The results of this study suggest that DHA may exert its antidepressant effect by translocating Gs alpha from lipid raft and potentiating the activity of adenylate cyclase. Importantly, the reduced Gs alpha in lipid raft by DHA is independent of disruption of lipid raft. Overall, the study provides partial preclinical evidence supporting a safe and effective therapy using DHA for depression. (C) 2015 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zhu, Zhuoran UNSPECIFIED UNSPECIFIED UNSPECIFIED Tan, Zhoubin UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Yan UNSPECIFIED UNSPECIFIED UNSPECIFIED Luo, Hongyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hu, Xinwu UNSPECIFIED UNSPECIFIED UNSPECIFIED Tang, Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Mu, Yangling UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Lanqiu UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-399781
DOI:	10.1016/j.nut.2015.02.012
Journal or Publication Title:	Nutrition
Volume:	31
Number:	7-8
Page Range:	S. 1025 - 1031
Date:	2015
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1873-1244
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYUNSATURATED FATTY-ACIDS; CHRONIC ANTIDEPRESSANT TREATMENT; BREAST-CANCER CELLS; POSTMORTEM BRAIN; SUICIDE VICTIMS; PLASMA-MEMBRANE; MESSENGER-RNA; DEPRESSION; PROTEIN; DOMAINS Multiple languages Nutrition & Dietetics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/39978