Universität zu Köln

Fusca, Debora, Schachtner, Joachim ORCID: 0000-0001-7268-0699 and Kloppenburg, Peter (2015). Colocalization of allatotropin and tachykinin-related peptides with classical transmitters in physiologically distinct subtypes of olfactory local interneurons in the cockroach (Periplaneta americana). J. Comp. Neurol., 523 (10). S. 1569 - 1587. HOBOKEN: WILEY-BLACKWELL. ISSN 1096-9861

Full text not available from this repository.

Abstract

In the insect antennal lobe different types of local interneurons mediate complex excitatory and inhibitory interactions between the glomerular pathways to structure the spatiotemporal representation of odors. Mass spectrometric and immunohistochemical studies have shown that in local interneurons classical neurotransmitters are likely to colocalize with a variety of substances that can potentially act as cotransmitters or neuromodulators. In the antennal lobe of the cockroach Periplaneta americana, gamma-aminobutyric acid (GABA) has been identified as the potential inhibitory transmitter of spiking type I local interneurons, whereas acetylcholine is most likely the excitatory transmitter of nonspiking type IIa1 local interneurons. This study used whole-cell patch clamp recordings combined with single-cell labeling and immunohistochemistry to test if the GABAergic type I local interneurons and the cholinergic type IIa1 local interneurons express allatotropin and tachykinin-related neuropeptides (TKRPs). These are two of the most abundant types of peptides in the insect antennal lobe. GABA-like and choline acetyltransferase (ChAT)-like immunoreactivity were used as markers for GABAergic and cholinergic neurons, respectively. About 50% of the GABA-like immunoreactive (-lir) spiking type I local interneurons were allatotropin-lir, and approximate to 40% of these neurons were TKRP-lir. About 20% of nonspiking ChAT-lir type IIa1 local interneurons were TKRP-lir. Our results suggest that in subpopulations of GABAergic and cholinergic local interneurons, allatotropin and TKRPs might act as cotransmitters or neuromodulators. To unequivocally assign neurotransmitters, cotransmitters, and neuromodulators to identified classes of antennal lobe neurons is an important step to deepen our understanding of information processing in the insect olfactory system. J. Comp. Neurol. 523:1569-1586, 2015. (c) 2015 Wiley Periodicals, Inc.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fusca, Debora UNSPECIFIED UNSPECIFIED UNSPECIFIED Schachtner, Joachim UNSPECIFIED orcid.org/0000-0001-7268-0699 UNSPECIFIED Kloppenburg, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-400762
DOI:	10.1002/cne.23757
Journal or Publication Title:	J. Comp. Neurol.
Volume:	523
Number:	10
Page Range:	S. 1569 - 1587
Date:	2015
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1096-9861
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DROSOPHILA ANTENNAL LOBE; GABA-LIKE IMMUNOREACTIVITY; MOTH HELIOTHIS-VIRESCENS; MANDUCA-SEXTA; INSECT BRAIN; NEUROPEPTIDE EXPRESSION; SCHISTOCERCA-GREGARIA; SYNAPTIC CONNECTIONS; PROJECTION NEURONS; NERVOUS-SYSTEM Multiple languages Neurosciences; Zoology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40076