Universität zu Köln

Asadi-Ghalehni, Majid, Ghaemmaghami, Mohamad, Klimka, Alexander, Javanmardi, Masoud ORCID: 0000-0001-8006-8619, Navari, Mohsen and Rasaee, Mohammad Javad (2015). Cancer immunotherapy by a recombinant phage vaccine displaying EGFR mimotope: an in vivo study. Immunopharmacol. Immunotoxicol., 37 (3). S. 274 - 280. ABINGDON: TAYLOR & FRANCIS LTD. ISSN 1532-2513

Full text not available from this repository.

Abstract

To date, several small molecule inhibitors and monoclonal-antibodies (like ICR-62) have been used to treat tumors over-expressing epidermal growth factor receptor (EGFR). However, the limitations associated with these conventional applications accentuate the necessity of alternative approaches. Mimotopes as compelling molecular tools could rationally be employed to circumvent these drawbacks. In the present study, an M13 phage displaying ICR-62 binding peptide mimotope is exploited as a vaccine candidate. It exhibited high affinity towards ICR62 and polyclonal anti-P-BSA antibodies. Following the mice immunization, phage-based mimotope vaccine induced humoral immunity. Elicited anti-EGFR mimotope antibodies were detected using ELISA method. Moreover, the phage vaccine was tested on the Lewis lung carcinoma mice model to investigate the prophylactic and therapeutic effects. The tumor volume was measured and recorded in different animal groups to evaluate the anti-tumor effects of the vaccine. Our data indicate that the reported phage-based mimotope could potentially elicit specific antibodies resulting in low titers of EGFR-specific antibodies and reduced tumor growth. However, in vivo experiments of prophylactic or therapeutic vaccination showed no specific advantage. Furthermore, phage-mimotope vaccine might be a promising approach in the field of cancer immunotherapy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Asadi-Ghalehni, Majid UNSPECIFIED UNSPECIFIED UNSPECIFIED Ghaemmaghami, Mohamad UNSPECIFIED UNSPECIFIED UNSPECIFIED Klimka, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Javanmardi, Masoud UNSPECIFIED orcid.org/0000-0001-8006-8619 UNSPECIFIED Navari, Mohsen UNSPECIFIED UNSPECIFIED UNSPECIFIED Rasaee, Mohammad Javad UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-402180
DOI:	10.3109/08923973.2015.1027917
Journal or Publication Title:	Immunopharmacol. Immunotoxicol.
Volume:	37
Number:	3
Page Range:	S. 274 - 280
Date:	2015
Publisher:	TAYLOR & FRANCIS LTD
Place of Publication:	ABINGDON
ISSN:	1532-2513
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GROWTH-FACTOR RECEPTOR; LEWIS LUNG-CARCINOMA; MONOCLONAL-ANTIBODY; FILAMENTOUS PHAGE; COLORECTAL-CANCER; EPITOPE; INDUCTION; THERAPY; PEPTIDE; TUMORS Multiple languages Immunology; Pharmacology & Pharmacy; Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40218