Haas, C., Hanson, E., Banemann, R., Bento, A. M., Berti, A., Carracedo, A., Courts, C., De Cock, G., Drobnic, K., Fleming, R., Franchi, C., Gomes, I., Hadzic, G., Harbison, S. A., Hjort, B., Hollard, C., Hoff-Olsen, P., Keyser, C., Kondili, A., Maronas, O., McCallum, N., Miniati, P., Morling, N., Niederstaetter, H., Noel, F., Parson, W., Porto, M. J., Roeder, A. D., Sauer, E., Schneider, P. M., Shanthan, G., Sijen, T., Court, D. Syndercombe, Turanska, M., van den Berge, M., Vennemann, M., Vidaki, A., Zatkalikova, L. and Ballantyne, J. (2015). RNA/DNA co-analysis from human skin and contact traces - results of a sixth collaborative EDNAP exercise. Forensic Sci. Int.-Genet., 16. S. 139 - 148. CLARE: ELSEVIER IRELAND LTD. ISSN 1878-0326

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Abstract

The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. Eight stains, a skin RNA dilution series and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 22 participating laboratories using RNA extraction or RNA/DNA co-extraction methods. Two sets of previously described skin-specific markers were used: skin1 pentaplex (LCE1C, LCE1D, LCE2D, IL1F7 and CCL27) and skin2 triplex (LOR, KRT9 and CDSN) in conjunction with a housekeeping gene, HKG, triplex (B2M, UBC and UCE). The laboratories used different chemistries and instrumentation. All laboratories were able to successfully isolate and detect mRNA in contact traces (e.g., human skin, palm-, hand-and fingerprints, clothing, car interiors, computer 2 accessories and electronic devices). The simultaneous extraction of RNA and DNA provides an opportunity for positive identification of the tissue source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling. The skin markers LCE1C and LOR and the housekeeping gene marker B2M were detected in the majority of contact traces. Detection of the other markers was inconsistent, possibly due to the low amounts and/or poor quality of the genetic material present in shed skin cells. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid/tissue identification method that can easily be combined with current STR typing technology. (c) 2015 Elsevier Ireland Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Haas, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hanson, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Banemann, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bento, A. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Berti, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carracedo, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Courts, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
De Cock, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Drobnic, K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fleming, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franchi, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gomes, I.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hadzic, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Harbison, S. A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hjort, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hollard, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoff-Olsen, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Keyser, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kondili, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maronas, O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
McCallum, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Miniati, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morling, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niederstaetter, H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Noel, F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parson, W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Porto, M. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roeder, A. D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sauer, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schneider, P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shanthan, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sijen, T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Court, D. SyndercombeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Turanska, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van den Berge, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vennemann, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vidaki, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zatkalikova, L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ballantyne, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-404697
DOI: 10.1016/j.fsigen.2015.01.002
Journal or Publication Title: Forensic Sci. Int.-Genet.
Volume: 16
Page Range: S. 139 - 148
Date: 2015
Publisher: ELSEVIER IRELAND LTD
Place of Publication: CLARE
ISSN: 1878-0326
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BODY-FLUID IDENTIFICATION; MESSENGER-RNA BIOMARKERS; POLYMERASE CHAIN-REACTION; VAGINAL SECRETIONS; MENSTRUAL BLOOD; SEMEN STAINS; CAPILLARY-ELECTROPHORESIS; FORENSIC IDENTIFICATION; EPITHELIAL-CELLS; SALIVA STAINSMultiple languages
Genetics & Heredity; Medicine, LegalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/40469

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