Universität zu Köln

Xue, Li, Cui, Haitao ORCID: 0000-0002-6343-1014, Buer, Benjamin, Vijayakumar, Vinod ORCID: 0000-0002-4923-5551, Delaux, Pierre-Marc ORCID: 0000-0002-6211-157X, Junkermann, Stefanie and Bucher, Marcel ORCID: 0000-0003-1680-9413 (2015). Network of GRAS Transcription Factors Involved in the Control of Arbuscule Development in Lotus japonicus. Plant Physiol., 167 (3). S. 854 - 922. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

Arbuscular mycorrhizal (AM) fungi, in symbiosis with plants, facilitate acquisition of nutrients from the soil to their host. After penetration, intracellular hyphae form fine-branched structures in cortical cells termed arbuscules, representing the major site where bidirectional nutrient exchange takes place between the host plant and fungus. Transcriptional mechanisms underlying this cellular reprogramming are still poorly understood. GRAS proteins are an important family of transcriptional regulators in plants, named after the first three members: GIBBERELLIC ACID-INSENSITIVE, REPRESSOR of GAI, and SCARECROW. Here, we show that among 45 transcription factors up-regulated in mycorrhizal roots of the legume Lotus japonicus, expression of a unique GRAS protein particularly increases in arbuscule-containing cells under low phosphate conditions and displays a phylogenetic pattern characteristic of symbiotic genes. Allelic rad1 mutants display a strongly reduced number of arbuscules, which undergo accelerated degeneration. In further studies, two RAD1-interacting proteins were identified. One of them is the closest homolog of Medicago truncatula, REDUCED ARBUSCULAR MYCORRHIZATION1 (RAM1), which was reported to regulate a glycerol-3-phosphate acyl transferase that promotes cutin biosynthesis to enhance hyphopodia formation. As in M. truncatula, the L. japonicus ram1 mutant lines show compromised AM colonization and stunted arbuscules. Our findings provide, to our knowledge, new insight into the transcriptional program underlying the host's response to AM colonization and propose a function of GRAS transcription factors including RAD1 and RAM1 during arbuscule development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Xue, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Cui, Haitao UNSPECIFIED orcid.org/0000-0002-6343-1014 UNSPECIFIED Buer, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED Vijayakumar, Vinod UNSPECIFIED orcid.org/0000-0002-4923-5551 UNSPECIFIED Delaux, Pierre-Marc UNSPECIFIED orcid.org/0000-0002-6211-157X UNSPECIFIED Junkermann, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucher, Marcel UNSPECIFIED orcid.org/0000-0003-1680-9413 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-407061
DOI:	10.1104/pp.114.255430
Journal or Publication Title:	Plant Physiol.
Volume:	167
Number:	3
Page Range:	S. 854 - 922
Date:	2015
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SYMBIOSIS SIGNALING PATHWAY; DEPENDENT PROTEIN-KINASE; MEDICAGO-TRUNCATULA; MYCORRHIZAL SYMBIOSIS; PHOSPHATE TRANSPORTER; GENE-EXPRESSION; ROOT ENDOSYMBIOSES; NODULE DEVELOPMENT; FUNGAL SYMBIOSIS; CALCIUM SPIKING Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/40706