Universität zu Köln

Luo, Dong, Fajol, Abul ORCID: 0000-0002-3202-5697, Umbach, Anja T., Noegel, Angelika A., Laufer, Stefan ORCID: 0000-0001-6952-1486, Lang, Florian and Foeller, Michael (2015). Influence of annexin A7 on insulin sensitivity of cellular glucose uptake. Pflugers Arch., 467 (4). S. 641 - 650. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1432-2013

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Abstract

Insulin sensitivity is decreased by prostaglandin E-2 (PGE(2)), a major product of cyclooxygenase (COX). As shown in erythrocytes, PGE(2) formation is inhibited by annexin A7. The present study defined the role of annexin A7 in glucose metabolism. Gene-targeted mice lacking annexin A7 (annexin7 (-/-)) were compared to wild-type mice (annexin7 (+/+)). The serum 6-Keto-prostaglandin-F-1 alpha (6-Keto-PGF(1)alpha) concentration was measured by ELISA and hepatic COX activity determined by an enzyme assay. Expression of COX-1, COX-2, prostaglandin E synthase, GLUT-4, and insulin receptor was determined by Western blotting. Glucose and insulin serum concentrations were analyzed following an intraperitoneal glucose load and glucose serum levels after intraperitoneal injection of insulin. Experiments were done without and with pretreatment of the mice with COX-inhibitor aspirin. The serum 6-Keto-PGF(1)alpha level and hepatic COX activity were significantly higher in annexin7 (-/-) than in annexin7 (+/+) mice. Hepatic COX-1 expression was higher in annexin7 (-/-) mice. Glucose tolerance was decreased in annexin7 (-/-) mice. Intraperitoneal insulin injection decreased the serum glucose level in both genotypes, an effect significantly less pronounced in annexin7 (-/-) mice. Glucose-induced insulin secretion was higher in annexin7 (-/-) mice. GLUT-4 expression in skeletal muscle from annexin7 (-/-) mice was reduced. Aspirin pretreatment lowered the increase in insulin concentration following glucose injection in both genotypes and virtually abrogated the differences in serum insulin between the genotypes. Aspirin pretreatment improved glucose tolerance in annexin7 (-/-) mice. In conclusion, annexin A7 influences insulin sensitivity of cellular glucose uptake and thus glucose tolerance. These effects depend on COX activity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Luo, Dong UNSPECIFIED UNSPECIFIED UNSPECIFIED Fajol, Abul UNSPECIFIED orcid.org/0000-0002-3202-5697 UNSPECIFIED Umbach, Anja T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Laufer, Stefan UNSPECIFIED orcid.org/0000-0001-6952-1486 UNSPECIFIED Lang, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Foeller, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-408655
DOI:	10.1007/s00424-014-1541-z
Journal or Publication Title:	Pflugers Arch.
Volume:	467
Number:	4
Page Range:	S. 641 - 650
Date:	2015
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1432-2013
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROSTATE-CANCER CELLS; PROSTAGLANDIN E-2; INDUCIBLE CYCLOOXYGENASE; CHROMAFFIN GRANULES; SODIUM-SALICYLATE; ANXA7 EXPRESSION; CALCIUM; RECEPTOR; SECRETION; PROTEINS Multiple languages Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40865