Universität zu Köln

Tennstaedt, Annette, Aswendt, Markus ORCID: 0000-0003-1423-0934, Adamczak, Joanna, Collienne, Ursel, Selt, Marion, Schneider, Gabriele, Henn, Nadine, Schaefer, Cordula, Lagouge, Marie, Wiedermann, Dirk, Kloppenburg, Peter and Hoehn, Mathias (2015). Human neural stem cell intracerebral grafts show spontaneous early neuronal differentiation after several weeks. Biomaterials, 44. S. 143 - 155. OXFORD: ELSEVIER SCI LTD. ISSN 1878-5905

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Abstract

Human neural stem cells (hNSCs) hold great promise for the treatment of neurological diseases. Considerable progress has been made to induce neural differentiation in the cell culture in vitro and upon transplantation in vivo [2] in order to explore restoration of damaged neuronal circuits. However, in vivo conventional strategies are limited to post mortem analysis. Here, we apply our developed first fate mapping platform to monitor neuronal differentiation in vivo by magnetic resonance imaging, bioluminescence imaging, and fluorescence imaging. Ferritin, Luciferase and GFP under neuronal-specific promoters for immature and mature neurons, respectively, were used to generate transgenic hNSCs. Differentiation-linked imaging reporter expression was validated in vitro. The time profile of spontaneous neuronal maturation after transplantation into mouse brain cortex demonstrated early neuronal differentiation within 6 weeks. Fully mature neurons expressing synaptogenesis were observed only after three months or longer. Our trimodal fate mapping strategy represents a unique non-invasive tool to monitor the time course of neuronal differentiation of transplanted stem cells in vivo. (C) 2014 The Authors. Published by Elsevier Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tennstaedt, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Aswendt, Markus UNSPECIFIED orcid.org/0000-0003-1423-0934 UNSPECIFIED Adamczak, Joanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Collienne, Ursel UNSPECIFIED UNSPECIFIED UNSPECIFIED Selt, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Henn, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaefer, Cordula UNSPECIFIED UNSPECIFIED UNSPECIFIED Lagouge, Marie UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiedermann, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloppenburg, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoehn, Mathias UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-411379
DOI:	10.1016/j.biomaterials.2014.12.038
Journal or Publication Title:	Biomaterials
Volume:	44
Page Range:	S. 143 - 155
Date:	2015
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1878-5905
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO DIFFERENTIATION; TRANSCRIPTIONAL REGULATION; LENTIVIRAL VECTORS; BRAIN-SLICES; MOUSE-BRAIN; RAT-BRAIN; STROKE; VIVO; GENE; REPORTER Multiple languages Engineering, Biomedical; Materials Science, Biomaterials Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41137