Universität zu Köln

Nitsche, Frank and Arndt, Hartmut ORCID: 0000-0003-2811-3595 (2015). Comparison of Similar Arctic and Antarctic Morphotypes of Heterotrophic Protists Regarding their Genotypes and Ecotypes. Protist, 166 (1). S. 42 - 58. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 1434-4610

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Abstract

The polar regions offer the opportunity to study possible diversification processes of spatially and temporally separated populations. We focused our study on similar morphotypes/species (e.g. species with the same morphology) of heterotrophic flagellates and ciliates originating from both, Antarctic and Arctic waters: 38 populations of six choanoflagellate morphospecies (Acanthocorbis unguiculata, Helgoeca nana, Diaphanoeca grandis, Savillea micropora, Stephanoeca apheles, Salpingoeca tuba), four other flagellate morphospecies (Cafeteria roenbergensis, Podomonas magma, Procryptobia sorokini, Protaspis sp.) and three ciliate morphospecies (Holosticha sp., Uronema marinum, Pseudocohnilembus persalinus). We analysed similarities and differences regarding their genotypes (SSU rDNA) and for several species regarding morphotypes and autecology (temperature and salinity tolerance). Most of the investigated polar protists were psychrophilic and showed a high salinity tolerance. Morphologically well defined acanthoecid choanoflagellates isolated from both poles showed the lowest intraspecific diversity (<0.5% p-distance). No intragenomic polymorphism of SSU rDNA within one individual and among clones from one population occurred. The way of dispersal for acanthoecid choanoflagellates still remains unclear. Even under extreme stress none of the examined cultures formed cysts. Single cell PCR appeared to be an appropriate method to investigate species not available as monoclonal cultures. As a prerequisite for barcoding, acanthoecid choanoflagellate species have a very low intraspecific variability regarding SSU rDNA. There was a clear correlation between autecological, morphological and molecular data sets, which may help interpreting molecular data from clone libraries or next generation sequencing. (C) 2014 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nitsche, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Arndt, Hartmut UNSPECIFIED orcid.org/0000-0003-2811-3595 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-412895
DOI:	10.1016/j.protis.2014.11.002
Journal or Publication Title:	Protist
Volume:	166
Number:	1
Page Range:	S. 42 - 58
Date:	2015
Publisher:	ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication:	JENA
ISSN:	1434-4610
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RNA GENE-SEQUENCES; WESTERN-AUSTRALIA; CRYPTIC DIVERSITY; WATER-COLUMN; SEA-ICE; SEDIMENTS; MARINE; POPULATIONS; SALINITY; BAY Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41289