Universität zu Köln

Schildgen, Verena, Pabst, Olga, Tillmann, Ramona-Liza, Luesebrink, Jessica, Schildgen, Oliver ORCID: 0000-0003-4297-9627, Ludwig, Corinna, Brockmann, Michael and Stoelben, Erich (2015). Low Frequency of EGFR Mutations in Pleural Mesothelioma Patients, Cologne, Germany. Appl. Immunohistochem., 23 (2). S. 118 - 126. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS. ISSN 1533-4058

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Abstract

EGFR mutations were previously found in patients suffering from peritoneal mesothelioma but have not yet been described in pleural mesothelioma. The aim of the present study was the identification of EGFR mutations in patients suffering from pleural mesothelioma. Pleural mesothelioma tissue from 31 patients was used to analyze possible mutations in the EGFR gene comprising the exons 18-21 with the codons 719, 768, 790, 858 + 861, 731 + 734, 785, 797 + 801, 831, and 868 with pyro-sequencing. The results indicate that 31 pleural mesothelioma patients show a wild-type EGFR gene when analyzing the codons D19, 768, 790, 858 + 861, 731 + 734, 785, 797 + 801, 831, and 868, whereas 2 patients have a mutation in the EGFR gene in codon 719. Sanger sequencing of the EGFR codon 785 was used for the determination of a polymorphism in the sequencing of tumor-free patients and pleural mesothelioma patients with a distribution of a wild-type homozygous sequence with guanine, a wild-type heterozygous sequence having guanine and adenine, a wild-type homozygous sequence with adenine, and a wild-type heterozygous sequence with adenine and guanine. Next, the identification of less EGFR mutations in the EGFR gene of the pleural mesothelioma an up to this time unknown polymorphism in the EGFR gene was identified which could be wrongly interpreted as a mutation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Pabst, Olga UNSPECIFIED UNSPECIFIED UNSPECIFIED Tillmann, Ramona-Liza UNSPECIFIED UNSPECIFIED UNSPECIFIED Luesebrink, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED Ludwig, Corinna UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmann, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoelben, Erich UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-413238
DOI:	10.1097/PDM.0b013e3182a3645e
Journal or Publication Title:	Appl. Immunohistochem.
Volume:	23
Number:	2
Page Range:	S. 118 - 126
Date:	2015
Publisher:	LIPPINCOTT WILLIAMS & WILKINS
Place of Publication:	PHILADELPHIA
ISSN:	1533-4058
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FACTOR RECEPTOR MUTATIONS; PERITONEAL MESOTHELIOMA; MALIGNANT MESOTHELIOMA; CELL-PROLIFERATION; GEFITINIB; TARGETS; ERK1/2 Multiple languages Anatomy & Morphology; Medical Laboratory Technology; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41323