Universität zu Köln

Placheta, Eva, Wood, Matthew D., Lafontaine, Christine, Liu, Edward H. ORCID: 0000-0003-4130-5786, Hendry, J. Michael, Angelov, Doychin N., Frey, Manfred, Gordon, Tessa and Borschel, Gregory H. (2015). Enhancement of Facial Nerve Motoneuron Regeneration through Cross-Face Nerve Grafts by Adding End-to-Side Sensory Axons. Plast. Reconstr. Surg., 135 (2). S. 460 - 472. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS. ISSN 1529-4242

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Abstract

Background: In unilateral facial palsy, cross-face nerve grafts are used for emotional facial reanimation. Facial nerve regeneration through the grafts takes several months, and the functional results are sometimes inadequate. Chronic denervation of the cross-face nerve graft results in incomplete nerve regeneration. The authors hypothesize that donor axons from regional sensory nerves will enhance facial motoneuron regeneration, improve axon regeneration, and improve the amplitude of facial muscle movement. Methods: In the rat model, a 30-mm nerve graft (right common peroneal nerve) was used as a cross-face nerve graft. The graft was coapted to the proximal stump of the transected right buccal branch of the facial nerve and the distal stumps of the transected left buccal and marginal mandibular branches. In one group, sensory occipital nerves were coapted end-to-side to the cross-face nerve graft. Regeneration of green fluorescent protein-positive axons was imaged in vivo in transgenic Thy1-green fluorescent protein rats, in which all neurons express green fluorescence. After 16 weeks, retrograde labeling of regenerated neurons and histomorphometric analysis of myelinated axons was performed. Functional outcomes were assessed with video analysis of whisker motion. Results: Pathway protection with sensory axons significantly enhanced motoneuron regeneration, as assessed by retrograde labeling, in vivo fluorescence imaging, and histomorphometry, and significantly improved whisker motion during video analysis. Conclusion: Sensory pathway protection of cross-face nerve grafts counteracts chronic denervation in nerve grafts and improves regeneration and functional outcomes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Placheta, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Wood, Matthew D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lafontaine, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Edward H. UNSPECIFIED orcid.org/0000-0003-4130-5786 UNSPECIFIED Hendry, J. Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Angelov, Doychin N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Frey, Manfred UNSPECIFIED UNSPECIFIED UNSPECIFIED Gordon, Tessa UNSPECIFIED UNSPECIFIED UNSPECIFIED Borschel, Gregory H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-413427
DOI:	10.1097/PRS.0000000000000893
Journal or Publication Title:	Plast. Reconstr. Surg.
Volume:	135
Number:	2
Page Range:	S. 460 - 472
Date:	2015
Publisher:	LIPPINCOTT WILLIAMS & WILKINS
Place of Publication:	PHILADELPHIA
ISSN:	1529-4242
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POOR FUNCTIONAL RECOVERY; SCHWANN-CELL DENERVATION; GRACILIS MUSCLE TRANSPLANTATION; Y-TUBE CONDUIT; MOTOR-NERVE; EXPERIMENTAL STRATEGIES; CONTRIBUTING FACTORS; MANUAL STIMULATION; NEW-MODEL; RAT Multiple languages Surgery Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41342